Determination of reactive oxygen species associated with the degeneration of dopaminergic neurons during dopamine metabolism

Mayumi Yamato, Wataru Kudo, Takeshi Shiba, Ken Iichi Yamada, Toshiaki Watanabe, Hideo Utsumi

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

Oxidative stress is believed to be an important mechanism underlying dopamine-induced neuronal damage. This study provides X-band electron spin resonance (ESR) spectroscopic evidence for reactive oxygen species (ROS) generation during dopamine metabolism. The authors induced excess dopamine metabolism in the mouse striatum by bathing it in tyramine-containing perfusate using microdialysis. The addition of tyramine to the perfusate raised the levels of extracellular dopamine and hydrogen peroxide significantly. The ESR signal from hydroxy-TEMPO decayed during tyramine perfusion and treatment with a monoamine-oxidase inhibitor or radical scavenger suppressed the signal decay. Decreases in the number of tyrosine hydroxylase-immunopositive fibres and in dopamine concentration after tyramine perfusion were observed. Moreover, the tyramine-perfused mice showed a marked methamphetamine-induced rotational response. Notably, these effects of tyramine were suppressed by the simultaneous perfusion of hydroxy-TEMPO. These findings indicate that the ROS generation, which was monitored by hydroxy-TEMPO, caused oxidative damage to the dopaminergic neurons.

元の言語英語
ページ(範囲)249-257
ページ数9
ジャーナルFree Radical Research
44
発行部数3
DOI
出版物ステータス出版済み - 3 1 2010

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Tyramine
Dopaminergic Neurons
Metabolism
Neurons
Reactive Oxygen Species
Dopamine
Perfusion
Electron Spin Resonance Spectroscopy
Paramagnetic resonance
Monoamine Oxidase Inhibitors
Oxidative stress
Methamphetamine
Microdialysis
Tyrosine 3-Monooxygenase
Hydrogen Peroxide
Oxidative Stress
Fibers
TEMPO

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

Determination of reactive oxygen species associated with the degeneration of dopaminergic neurons during dopamine metabolism. / Yamato, Mayumi; Kudo, Wataru; Shiba, Takeshi; Yamada, Ken Iichi; Watanabe, Toshiaki; Utsumi, Hideo.

:: Free Radical Research, 巻 44, 番号 3, 01.03.2010, p. 249-257.

研究成果: ジャーナルへの寄稿記事

Yamato, Mayumi ; Kudo, Wataru ; Shiba, Takeshi ; Yamada, Ken Iichi ; Watanabe, Toshiaki ; Utsumi, Hideo. / Determination of reactive oxygen species associated with the degeneration of dopaminergic neurons during dopamine metabolism. :: Free Radical Research. 2010 ; 巻 44, 番号 3. pp. 249-257.
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