Determination of tetrahydrocannabinolic acid—carrier protein conjugate by matrix‐assisted laser desorption/ionization mass spectrometry and antibody formation

Yuri Goto, Yumi Shima, Satoshi Morimoto, Yukihiro Shoyama, Hiroki Murakami, Akihiko Kusai, Kazutetsu Nojima

研究成果: Contribution to journalArticle査読

43 被引用数 (Scopus)

抄録

In order to form the monoclonal antibody against tetrahydrocannabinolic acid (THCA), THCA was coupled with bovine serum albumin (BSA) or human serum albumin (HSA) using β‐alanine as a linker or without β‐alanine to produce individual antigen conjugates. The number of haptens contained in the antigen conjugates was determined by matrix‐assisted laser desorption ionization mass spectrometry using BSA or HSA as an internal standard. The formation of monoclonal antibody is also discussed.

本文言語英語
ページ(範囲)668-671
ページ数4
ジャーナルOrganic Mass Spectrometry
29
11
DOI
出版ステータス出版済み - 1 1 1994

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 器械工学

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