Determination of the rate of rRNA synthesis in Xenopus laevis triploid embryos produced by low‐temperature treatment

K. Tashiro, Y. Misumi, K. Shiokawa, K. Yamana

研究成果: ジャーナルへの寄稿記事

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Triploid embryos of Xenopus laevis were obtained by cold‐temperature shocking of the fertilized eggs, and the rate of the ribosonial RNA (rRNA) synthesis was determined for comparison with that in diploid embryos. For this purpose, both triploid and diploid embryos were dissociated into cells at the neu‐rula stage, and then labeled with (3H)uridine for varying lengths of time. The rate of rRNA synthesis, as estimated after determination of (3H)UTP specific radioactivity and the total label incorporation into the purified rRNA, was about 0.1 pg/cell/hr for both diploid and triploid embryo cells. Nuclei of triploid embryo cells contained three nucleoli of apparently similar sizes—an indication of the functioning of all the three rRNA gene clusters to a more or less similar extent. Also, rates of synthesis of 4S RNA and 5S RNA were determined: Both rates did not change appreciably between triploid and diploid embryo cells. Based on these results, it appears that transcription of these redundant genes occurs at a constant rate on a per cell basis irrespective of the presence of 1.5 times as many genes as the control.

元の言語英語
ページ(範囲)489-495
ページ数7
ジャーナルJournal of Experimental Zoology
225
発行部数3
DOI
出版物ステータス出版済み - 3 1983

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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