Determination of thiosulfate in body fluids by GC and GC/MS

S. Kage, T. Nagata, K. Kudo

研究成果: Contribution to journalArticle査読

41 被引用数 (Scopus)

抄録

A simple and sensitive method to determine thiosulfate in human blood and urine was devised. Thiosulfate was first alkylated with pentafluorobenzyl bromide, with L-ascorbic acid as the stabilizer and sodium chloride as the catalyst. The alkylated thiosulfate was oxidized with iodine to obtain bis(pentafluorobenzyl)disulfide, which was then subjected to gas chromatography and gas chromatography/mass spectrometry. Mass fragmentography was used to identify the disulfide, and gas chromatography with an electron capture detector was used for quantitative determination. The lower limit of detection was 0.003 μmol/mL.

本文言語英語
ページ(範囲)148-150
ページ数3
ジャーナルJournal of Analytical Toxicology
15
3
DOI
出版ステータス出版済み - 1 1 1991

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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