Determination of total and pancreatic amylase activities in human blood by use of microchip electrophoresis

Eiki Maeda, Masatoshi Kataoka, Yasuo Shinohara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

1 被引用数 (Scopus)

抄録

In this study, a high-performance determination system for isoenzyme activities of human a-amylase was developed using microchip electrophoresis (ME) with a plastic chip. The combination of ME for substrate/hydrolysate separation and immunomhibition method for differentiation of isoenzymes using anti human salivary amylase (S-AMY) monoclonal antibody, Tu88E8, realized the highly selective determination of amylase isoenzyme activities even in a complex matrix such as a blood sample. This paper demonstrates that this system enables us to determine amylase isoenzyme activities with easy operation and high accuracy, and it will be applicable to clinical diagnosis for acute pancreatic disorders.

本文言語英語
ホスト出版物のタイトルProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
編集者Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
出版社Chemical and Biological Microsystems Society
ページ65-67
ページ数3
ISBN(電子版)9780979806407
出版ステータス出版済み - 2007
外部発表はい
イベント11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, フランス
継続期間: 10 7 200710 11 2007

出版物シリーズ

名前Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

会議

会議11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Countryフランス
CityParis
Period10/7/0710/11/07

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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