Development and characterization of simple sequence repeat DNA markers for Zelkova serrata

E. Fukatsu, K. Isoda, T. Hirao, M. Takahashi, A. Watanabe

研究成果: Contribution to journalArticle査読

17 被引用数 (Scopus)

抄録

We developed 14 microsatellite loci from an enriched genomic DNA library of a broad-leaved deciduous tree, Zelkova serrata. Of 198 clones from the library, 112 contained microsatellite repeat regions. The M13-tailed primer method was used for economy. Sequence-specific primer pairs were designed for 58 of 76 candidate clones. Fourteen of these primer pairs successfully amplified polymorphic single loci among 34 individuals collected from the Kanto breeding region in Japan. The expected heterozygosity for the 14 microsatellite markers ranged from 0.378 to 0.876, suggesting that these will prove valuable for breeding and ecological studies on Z. serrata.

本文言語英語
ページ(範囲)378-380
ページ数3
ジャーナルMolecular Ecology Notes
5
2
DOI
出版ステータス出版済み - 6 1 2005
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 生態学
  • 生化学、遺伝学、分子生物学(全般)

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