Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’

Mitsukazu Sakata, Mari Seno, Hiroaki Matsusaka, Kiyomi Takahashi, Yuki Nakamura, Yoshiyuki Yamagata, Enrique R. Angeles, Toshihiro Mochizuki, Toshihiro Kumamaru, Masao Sato, Akiko Enomoto, Kosuke Tashiro, Satoru Kuhara, Hikaru Satoh, Atsushi Yoshimura

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.

元の言語英語
ページ(範囲)425-433
ページ数9
ジャーナルBreeding Science
66
発行部数3
DOI
出版物ステータス出版済み - 1 1 2016

Fingerprint

high-yielding varieties
Oils
Embryonic Structures
lipid content
rice
mutants
milling byproducts
N-methyl-N-nitrosourea
triacylglycerols
Breeding
chromosomes
rice bran oil
loci
Triglycerides
rice bran
breeding
ova
Methylnitrosourea
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

これを引用

Sakata, M., Seno, M., Matsusaka, H., Takahashi, K., Nakamura, Y., Yamagata, Y., ... Yoshimura, A. (2016). Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’. Breeding Science, 66(3), 425-433. https://doi.org/10.1270/jsbbs.15135

Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’. / Sakata, Mitsukazu; Seno, Mari; Matsusaka, Hiroaki; Takahashi, Kiyomi; Nakamura, Yuki; Yamagata, Yoshiyuki; Angeles, Enrique R.; Mochizuki, Toshihiro; Kumamaru, Toshihiro; Sato, Masao; Enomoto, Akiko; Tashiro, Kosuke; Kuhara, Satoru; Satoh, Hikaru; Yoshimura, Atsushi.

:: Breeding Science, 巻 66, 番号 3, 01.01.2016, p. 425-433.

研究成果: ジャーナルへの寄稿記事

Sakata, M, Seno, M, Matsusaka, H, Takahashi, K, Nakamura, Y, Yamagata, Y, Angeles, ER, Mochizuki, T, Kumamaru, T, Sato, M, Enomoto, A, Tashiro, K, Kuhara, S, Satoh, H & Yoshimura, A 2016, 'Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’', Breeding Science, 巻. 66, 番号 3, pp. 425-433. https://doi.org/10.1270/jsbbs.15135
Sakata, Mitsukazu ; Seno, Mari ; Matsusaka, Hiroaki ; Takahashi, Kiyomi ; Nakamura, Yuki ; Yamagata, Yoshiyuki ; Angeles, Enrique R. ; Mochizuki, Toshihiro ; Kumamaru, Toshihiro ; Sato, Masao ; Enomoto, Akiko ; Tashiro, Kosuke ; Kuhara, Satoru ; Satoh, Hikaru ; Yoshimura, Atsushi. / Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’. :: Breeding Science. 2016 ; 巻 66, 番号 3. pp. 425-433.
@article{be70fb83758444b084fc699767bf7ca2,
title = "Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’",
abstract = "Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.",
author = "Mitsukazu Sakata and Mari Seno and Hiroaki Matsusaka and Kiyomi Takahashi and Yuki Nakamura and Yoshiyuki Yamagata and Angeles, {Enrique R.} and Toshihiro Mochizuki and Toshihiro Kumamaru and Masao Sato and Akiko Enomoto and Kosuke Tashiro and Satoru Kuhara and Hikaru Satoh and Atsushi Yoshimura",
year = "2016",
month = "1",
day = "1",
doi = "10.1270/jsbbs.15135",
language = "English",
volume = "66",
pages = "425--433",
journal = "Breeding Science",
issn = "1344-7610",
publisher = "Japanese Society of Breeding",
number = "3",

}

TY - JOUR

T1 - Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’

AU - Sakata, Mitsukazu

AU - Seno, Mari

AU - Matsusaka, Hiroaki

AU - Takahashi, Kiyomi

AU - Nakamura, Yuki

AU - Yamagata, Yoshiyuki

AU - Angeles, Enrique R.

AU - Mochizuki, Toshihiro

AU - Kumamaru, Toshihiro

AU - Sato, Masao

AU - Enomoto, Akiko

AU - Tashiro, Kosuke

AU - Kuhara, Satoru

AU - Satoh, Hikaru

AU - Yoshimura, Atsushi

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.

AB - Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.

UR - http://www.scopus.com/inward/record.url?scp=84976482186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976482186&partnerID=8YFLogxK

U2 - 10.1270/jsbbs.15135

DO - 10.1270/jsbbs.15135

M3 - Article

VL - 66

SP - 425

EP - 433

JO - Breeding Science

JF - Breeding Science

SN - 1344-7610

IS - 3

ER -