抄録
Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 425-433 |
| ページ数 | 9 |
| ジャーナル | Breeding Science |
| 巻 | 66 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2016 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Genetics
- Plant Science
これを引用
Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’. / Sakata, Mitsukazu; Seno, Mari; Matsusaka, Hiroaki; Takahashi, Kiyomi; Nakamura, Yuki; Yamagata, Yoshiyuki; Angeles, Enrique R.; Mochizuki, Toshihiro; Kumamaru, Toshihiro; Sato, Masao; Enomoto, Akiko; Tashiro, Kosuke; Kuhara, Satoru; Satoh, Hikaru; Yoshimura, Atsushi.
：: Breeding Science, 巻 66, 番号 3, 01.01.2016, p. 425-433.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’
AU - Sakata, Mitsukazu
AU - Seno, Mari
AU - Matsusaka, Hiroaki
AU - Takahashi, Kiyomi
AU - Nakamura, Yuki
AU - Yamagata, Yoshiyuki
AU - Angeles, Enrique R.
AU - Mochizuki, Toshihiro
AU - Kumamaru, Toshihiro
AU - Sato, Masao
AU - Enomoto, Akiko
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Satoh, Hikaru
AU - Yoshimura, Atsushi
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.
AB - Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar ‘Mizuhochikara’ with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, ‘Mizuhochikara’. MGE13 was agronomically similar to ‘Mizuhochikara’ and would be useful for breeding for improved oil content.
UR - http://www.scopus.com/inward/record.url?scp=84976482186&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84976482186&partnerID=8YFLogxK
U2 - 10.1270/jsbbs.15135
DO - 10.1270/jsbbs.15135
M3 - Article
VL - 66
SP - 425
EP - 433
JO - Breeding Science
JF - Breeding Science
SN - 1344-7610
IS - 3
ER -