Ozone has the capability of sterilization and deodorization due to high oxidation power. It is also effective for the conservation of perishable foods and purification of water. However, ozone has a disadvantage, that is, conservation of ozone is difficult because it reacts to oxygen. Recently, ozone-contained ice is taken attention for the purpose of its conservation. The use of ozone-contained ice seems to keep food fresher when we conserve and transport perishable foods due to the effects of cooling and sterilization of ozone-contained ice. In the present study, we have developed an ozone-contained ice making machine employing pressurized air tight containers with commercially available size. And the performance evaluation of the system is also carried out. Furthermore, we investigated the sterilization effect of ozone-contained ice on conservation of fish. It was seen that ozone-contained ice is effective for sterilization of surface of fish.