Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

Shota Shimokihara, Shunya Shitara, Ryo Oyobiki, Takeshi Watanabe, Yasuaki Einaga, Yoshinori Matsumoto, Kei Fujiwara, Kenichi Horisawa, Nobuhide Doi

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.

元の言語英語
ホスト出版物のタイトルMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版者Chemical and Biological Microsystems Society
ページ1302-1304
ページ数3
ISBN(電子版)9780979806483
出版物ステータス出版済み - 1 1 2015
イベント19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, 大韓民国
継続期間: 10 25 201510 29 2015

出版物シリーズ

名前MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

その他

その他19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
大韓民国
Gyeongju
期間10/25/1510/29/15

Fingerprint

Screening
Throughput
Microfluidics
Biological fuel cells
Sorting
Biosensors
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

これを引用

Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Matsumoto, Y., ... Doi, N. (2015). Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. : MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1302-1304). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. / Shimokihara, Shota; Shitara, Shunya; Oyobiki, Ryo; Watanabe, Takeshi; Einaga, Yasuaki; Matsumoto, Yoshinori; Fujiwara, Kei; Horisawa, Kenichi; Doi, Nobuhide.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 1302-1304 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

研究成果: 著書/レポートタイプへの貢献会議での発言

Shimokihara, S, Shitara, S, Oyobiki, R, Watanabe, T, Einaga, Y, Matsumoto, Y, Fujiwara, K, Horisawa, K & Doi, N 2015, Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. : MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1302-1304, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, 大韓民国, 10/25/15.
Shimokihara S, Shitara S, Oyobiki R, Watanabe T, Einaga Y, Matsumoto Y その他. Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. : MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 1302-1304. (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Shimokihara, Shota ; Shitara, Shunya ; Oyobiki, Ryo ; Watanabe, Takeshi ; Einaga, Yasuaki ; Matsumoto, Yoshinori ; Fujiwara, Kei ; Horisawa, Kenichi ; Doi, Nobuhide. / Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 1302-1304 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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