Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin

Supaluk Krittanai, Tharita Kitisripanya, Orapin Udomsin, Hiroyuki Tanaka, Seiichi Sakamoto, Thaweesak Juengwatanatrakul, Waraporn Putalun

研究成果: ジャーナルへの寄稿記事

抄録

Pueraria candollei or White Kwao Krua (Leguminosae) is an indigenous plant in Thailand which has long been used in Thai traditional medicine. The tuberous root of this plant is widely used for rejuvenation, particularly in elder women. Among the bioactive compounds in P. candollei, miroestrol and puerarin exhibit estrogenic activity. This study aims to develop an immunochromatographic strip (ICS) with a colloidal gold-based detection system for the simultaneous detection of miroestrol and puerarin in a one-step analysis. The developed method is sensitive and specific for the detection of miroestrol and puerarin in raw materials and marketed products. The detection limits of miroestrol and puerarin were 0.15 and 4.5 μg, respectively. In addition, the results from the developed ICS were confirmed with an enzyme-linked immunosorbent assay and presented a good correlation between these two methods. This is the first report on the development of an ICS that can detect miroestrol and puerarin in one step. The developed ICS provides a simplified method for the detection of miroestrol and puerarin in P. candollei and Pueraria spp.

元の言語英語
記事番号e4330
ジャーナルBiomedical Chromatography
32
発行部数11
DOI
出版物ステータス出版済み - 11 1 2018

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Gold Colloid
Nanoparticles
Pueraria
Rejuvenation
Immunosorbents
Plant Roots
Traditional Medicine
Thailand
Fabaceae
Medicine
Limit of Detection
puerarin
miroestrol
Assays
Raw materials
Enzyme-Linked Immunosorbent Assay
Enzymes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

これを引用

Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin. / Krittanai, Supaluk; Kitisripanya, Tharita; Udomsin, Orapin; Tanaka, Hiroyuki; Sakamoto, Seiichi; Juengwatanatrakul, Thaweesak; Putalun, Waraporn.

:: Biomedical Chromatography, 巻 32, 番号 11, e4330, 01.11.2018.

研究成果: ジャーナルへの寄稿記事

Krittanai, Supaluk ; Kitisripanya, Tharita ; Udomsin, Orapin ; Tanaka, Hiroyuki ; Sakamoto, Seiichi ; Juengwatanatrakul, Thaweesak ; Putalun, Waraporn. / Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin. :: Biomedical Chromatography. 2018 ; 巻 32, 番号 11.
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AU - Tanaka, Hiroyuki

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AU - Juengwatanatrakul, Thaweesak

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