Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel

Qian Cui, Hiroyuki Tanaka, Yukihiro Shoyama, Hao Ting Ye, Fang Li, En Wei Tian, Ying Song Wu, Zhi Chao

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)

抄録

Introduction: Paclitaxel (Tax) is a diterpene alkaloid isolated from Taxus species and has proved clinically effective in treating a number of malignancies. Current quantitative analytical methods for Tax such as high-performance liquid chromatography (HPLC) often involve complicated sample preparation procedures with low recovery rates. Objective: To establish a rapid and sensitive time-resolved fluoroimmunoassay (TRFIA) for measuring Tax in Taxus materials with convenient sample preparation and a high recovery rate. Methods: Rabbit anti-mouse IgG was coated onto a 96-well microplate, which was then incubated with standard solutions of Tax and anti-Tax monoclonal antibody 3A3. A Eu3+-labelled conjugate of Tax and human serum albumin was used as the tracer. The luminescent system was enhanced with a solution containing 2-naphthoyltrifluoroacetone. Results: The established TRFIA showed a linear response within the Tax concentration range of 3.2 to 80 ng/mL, with a limit of detection of 1.4 ng/mL. The intra- and inter-assay coefficient of variation of the assay was 9.6% and 9.7%, respectively, with an average recovery rate from spiked samples of 108.5%. Tax contents in Taxus samples were determined using both the established TRFIA system and a previously established enzyme-linked immunosorbent (ELISA), and the results of two assays were well correlated. Conclusion: This TRFIA system shows a high sensitivity, precision and accuracy for detection of Tax. This assay, which is convenient and less time-consuming, allows rapid analysis of Tax and provides another option for Tax measurement for quality control of Taxus materials and products.

本文言語英語
ページ(範囲)284-289
ページ数6
ジャーナルPhytochemical Analysis
29
3
DOI
出版ステータス出版済み - 5月 1 2018

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 食品科学
  • 生化学
  • 分子医療
  • 植物科学
  • 創薬
  • 補完代替医療

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