Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel

Qian Cui, Hiroyuki Tanaka, Yukihiro Shoyama, Hao Ting Ye, Fang Li, En Wei Tian, Ying Song Wu, Zhi Chao

研究成果: ジャーナルへの寄稿記事

抄録

Introduction: Paclitaxel (Tax) is a diterpene alkaloid isolated from Taxus species and has proved clinically effective in treating a number of malignancies. Current quantitative analytical methods for Tax such as high-performance liquid chromatography (HPLC) often involve complicated sample preparation procedures with low recovery rates. Objective: To establish a rapid and sensitive time-resolved fluoroimmunoassay (TRFIA) for measuring Tax in Taxus materials with convenient sample preparation and a high recovery rate. Methods: Rabbit anti-mouse IgG was coated onto a 96-well microplate, which was then incubated with standard solutions of Tax and anti-Tax monoclonal antibody 3A3. A Eu3+-labelled conjugate of Tax and human serum albumin was used as the tracer. The luminescent system was enhanced with a solution containing 2-naphthoyltrifluoroacetone. Results: The established TRFIA showed a linear response within the Tax concentration range of 3.2 to 80 ng/mL, with a limit of detection of 1.4 ng/mL. The intra- and inter-assay coefficient of variation of the assay was 9.6% and 9.7%, respectively, with an average recovery rate from spiked samples of 108.5%. Tax contents in Taxus samples were determined using both the established TRFIA system and a previously established enzyme-linked immunosorbent (ELISA), and the results of two assays were well correlated. Conclusion: This TRFIA system shows a high sensitivity, precision and accuracy for detection of Tax. This assay, which is convenient and less time-consuming, allows rapid analysis of Tax and provides another option for Tax measurement for quality control of Taxus materials and products.

元の言語英語
ページ(範囲)284-289
ページ数6
ジャーナルPhytochemical Analysis
29
発行部数3
DOI
出版物ステータス出版済み - 5 1 2018

Fingerprint

Fluoroimmunoassay
paclitaxel
Taxus
taxes
Paclitaxel
Taxation
Assays
assays
Immunosorbents
Diterpenes
Alkaloids
Serum Albumin
Quality Control
Recovery
Limit of Detection
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Monoclonal Antibodies
Rabbits
sampling

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

これを引用

Cui, Q., Tanaka, H., Shoyama, Y., Ye, H. T., Li, F., Tian, E. W., ... Chao, Z. (2018). Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel. Phytochemical Analysis, 29(3), 284-289. https://doi.org/10.1002/pca.2741

Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel. / Cui, Qian; Tanaka, Hiroyuki; Shoyama, Yukihiro; Ye, Hao Ting; Li, Fang; Tian, En Wei; Wu, Ying Song; Chao, Zhi.

:: Phytochemical Analysis, 巻 29, 番号 3, 01.05.2018, p. 284-289.

研究成果: ジャーナルへの寄稿記事

Cui, Q, Tanaka, H, Shoyama, Y, Ye, HT, Li, F, Tian, EW, Wu, YS & Chao, Z 2018, 'Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel', Phytochemical Analysis, 巻. 29, 番号 3, pp. 284-289. https://doi.org/10.1002/pca.2741
Cui Q, Tanaka H, Shoyama Y, Ye HT, Li F, Tian EW その他. Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel. Phytochemical Analysis. 2018 5 1;29(3):284-289. https://doi.org/10.1002/pca.2741
Cui, Qian ; Tanaka, Hiroyuki ; Shoyama, Yukihiro ; Ye, Hao Ting ; Li, Fang ; Tian, En Wei ; Wu, Ying Song ; Chao, Zhi. / Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel. :: Phytochemical Analysis. 2018 ; 巻 29, 番号 3. pp. 284-289.
@article{7dec8a57681440f78dd9cf8f48150064,
title = "Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel",
abstract = "Introduction: Paclitaxel (Tax) is a diterpene alkaloid isolated from Taxus species and has proved clinically effective in treating a number of malignancies. Current quantitative analytical methods for Tax such as high-performance liquid chromatography (HPLC) often involve complicated sample preparation procedures with low recovery rates. Objective: To establish a rapid and sensitive time-resolved fluoroimmunoassay (TRFIA) for measuring Tax in Taxus materials with convenient sample preparation and a high recovery rate. Methods: Rabbit anti-mouse IgG was coated onto a 96-well microplate, which was then incubated with standard solutions of Tax and anti-Tax monoclonal antibody 3A3. A Eu3+-labelled conjugate of Tax and human serum albumin was used as the tracer. The luminescent system was enhanced with a solution containing 2-naphthoyltrifluoroacetone. Results: The established TRFIA showed a linear response within the Tax concentration range of 3.2 to 80 ng/mL, with a limit of detection of 1.4 ng/mL. The intra- and inter-assay coefficient of variation of the assay was 9.6{\%} and 9.7{\%}, respectively, with an average recovery rate from spiked samples of 108.5{\%}. Tax contents in Taxus samples were determined using both the established TRFIA system and a previously established enzyme-linked immunosorbent (ELISA), and the results of two assays were well correlated. Conclusion: This TRFIA system shows a high sensitivity, precision and accuracy for detection of Tax. This assay, which is convenient and less time-consuming, allows rapid analysis of Tax and provides another option for Tax measurement for quality control of Taxus materials and products.",
author = "Qian Cui and Hiroyuki Tanaka and Yukihiro Shoyama and Ye, {Hao Ting} and Fang Li and Tian, {En Wei} and Wu, {Ying Song} and Zhi Chao",
year = "2018",
month = "5",
day = "1",
doi = "10.1002/pca.2741",
language = "English",
volume = "29",
pages = "284--289",
journal = "Phytochemical Analysis",
issn = "0958-0344",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel

AU - Cui, Qian

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

AU - Ye, Hao Ting

AU - Li, Fang

AU - Tian, En Wei

AU - Wu, Ying Song

AU - Chao, Zhi

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Introduction: Paclitaxel (Tax) is a diterpene alkaloid isolated from Taxus species and has proved clinically effective in treating a number of malignancies. Current quantitative analytical methods for Tax such as high-performance liquid chromatography (HPLC) often involve complicated sample preparation procedures with low recovery rates. Objective: To establish a rapid and sensitive time-resolved fluoroimmunoassay (TRFIA) for measuring Tax in Taxus materials with convenient sample preparation and a high recovery rate. Methods: Rabbit anti-mouse IgG was coated onto a 96-well microplate, which was then incubated with standard solutions of Tax and anti-Tax monoclonal antibody 3A3. A Eu3+-labelled conjugate of Tax and human serum albumin was used as the tracer. The luminescent system was enhanced with a solution containing 2-naphthoyltrifluoroacetone. Results: The established TRFIA showed a linear response within the Tax concentration range of 3.2 to 80 ng/mL, with a limit of detection of 1.4 ng/mL. The intra- and inter-assay coefficient of variation of the assay was 9.6% and 9.7%, respectively, with an average recovery rate from spiked samples of 108.5%. Tax contents in Taxus samples were determined using both the established TRFIA system and a previously established enzyme-linked immunosorbent (ELISA), and the results of two assays were well correlated. Conclusion: This TRFIA system shows a high sensitivity, precision and accuracy for detection of Tax. This assay, which is convenient and less time-consuming, allows rapid analysis of Tax and provides another option for Tax measurement for quality control of Taxus materials and products.

AB - Introduction: Paclitaxel (Tax) is a diterpene alkaloid isolated from Taxus species and has proved clinically effective in treating a number of malignancies. Current quantitative analytical methods for Tax such as high-performance liquid chromatography (HPLC) often involve complicated sample preparation procedures with low recovery rates. Objective: To establish a rapid and sensitive time-resolved fluoroimmunoassay (TRFIA) for measuring Tax in Taxus materials with convenient sample preparation and a high recovery rate. Methods: Rabbit anti-mouse IgG was coated onto a 96-well microplate, which was then incubated with standard solutions of Tax and anti-Tax monoclonal antibody 3A3. A Eu3+-labelled conjugate of Tax and human serum albumin was used as the tracer. The luminescent system was enhanced with a solution containing 2-naphthoyltrifluoroacetone. Results: The established TRFIA showed a linear response within the Tax concentration range of 3.2 to 80 ng/mL, with a limit of detection of 1.4 ng/mL. The intra- and inter-assay coefficient of variation of the assay was 9.6% and 9.7%, respectively, with an average recovery rate from spiked samples of 108.5%. Tax contents in Taxus samples were determined using both the established TRFIA system and a previously established enzyme-linked immunosorbent (ELISA), and the results of two assays were well correlated. Conclusion: This TRFIA system shows a high sensitivity, precision and accuracy for detection of Tax. This assay, which is convenient and less time-consuming, allows rapid analysis of Tax and provides another option for Tax measurement for quality control of Taxus materials and products.

UR - http://www.scopus.com/inward/record.url?scp=85045150460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045150460&partnerID=8YFLogxK

U2 - 10.1002/pca.2741

DO - 10.1002/pca.2741

M3 - Article

C2 - 29266486

AN - SCOPUS:85045150460

VL - 29

SP - 284

EP - 289

JO - Phytochemical Analysis

JF - Phytochemical Analysis

SN - 0958-0344

IS - 3

ER -