Development of a DNA array for the simple identification of major filamentous fungi in the beverage manufacturing environment

Fuyuki Aoyama, Takahisa Miyamoto

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Filamentous fungi were isolated from the indoor environment of a soft drink manufacturing plant and ordinary residences. The isolated strains were identified based on morphological observation and the nucleotide sequences of the region near the D2 region of the 26S rDNA. Three genera (Aspergillus, Penicillium, and Cladosporium) accounted for 48.1% of the fungal strains detected in the manufacturing plant and 75.3% in residences. A DNA array for identification of 15 genera and 26 species of filamentous fungi that were most frequently isolated from the manufacturing plant was developed. Genus- and species-specific probes with 13- to 20-mer were designed on the basis of the nucleotide sequences in the D2 region. The probes were affixed to a microscope slide after modifying an amino group at the 5′ or 3′ end. To prevent erroneous identification, 2 or 3 probes were designed for each of the target genera and species. The developed DNA array method correctly identified 9 genera (Alternaria, Aureobasidium, Cladosporium, Curvularia, Exophiala, Fusarium, Penicillium, Phoma, and Trichoderma) and 26 species belonging to 6 genera (Aspergillus, Neosartorya, Byssochlamys, Talaromyces, Paecilomyces, and Purpureocillium) in the strains isolated from the indoor environment. Identification results obtained by this DNA array method of fungi isolated from the manufacturing plant were consistent with those by the conventional method.

元の言語英語
ページ(範囲)161-172
ページ数12
ジャーナルBiocontrol Science
21
発行部数3
DOI
出版物ステータス出版済み - 1 1 2016

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Beverages
Oligonucleotide Array Sequence Analysis
Fungi
Cladosporium
Penicillium
Aspergillus
Byssochlamys
Neosartorya
Talaromyces
Exophiala
Paecilomyces
Carbonated Beverages
Alternaria
Trichoderma
Fusarium
Ribosomal DNA
Observation
Manufacturing and Industrial Facilities

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

これを引用

Development of a DNA array for the simple identification of major filamentous fungi in the beverage manufacturing environment. / Aoyama, Fuyuki; Miyamoto, Takahisa.

:: Biocontrol Science, 巻 21, 番号 3, 01.01.2016, p. 161-172.

研究成果: ジャーナルへの寄稿記事

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