Development of a fluorescence peptide chip for the detection of caspase activity

Aishan Han, Tatsuhiko Sonoda, Jeong Hun Kang, Masaharu Murata, Takuro Niiidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Proteases play a key role in cell functions, and it is very important to monitor their activities for drug screening and diagnosis of diseases. In the present study, a new class of fluorescence probe, into which a fluorophore and a quencher have been introduced, was developed and applied to the on-chip detection of caspase-3 activity. This probe is non fluorescent in the absence of caspase-3. However, when it is treated with active caspase-3, the fluorescence intensity increases dependent on the caspase-3 activity due to the cleavage of the quencher-containing moiety on a glass slide. This caspase-dependent increase in the fluorescence intensity was also detected when the glass slide immobilizing the probe peptide was treated with cell lysate stimulated by staurosporine (STP), which is an apoptosis-inducing agent. On the other hand, such an increase was not detected in the case of control cell lysate without STP-stimulation. The developed system is a rapid and sensitive method and is useful for the direct measurement of protease activity on a glass array.

元の言語英語
ページ(範囲)21-25
ページ数5
ジャーナルCombinatorial Chemistry and High Throughput Screening
9
発行部数1
DOI
出版物ステータス出版済み - 1 1 2006

Fingerprint

Caspases
Caspase 3
Peptides
Fluorescence
Glass
Staurosporine
Peptide Hydrolases
Fluorophores
Cell death
Preclinical Drug Evaluations
Screening
Apoptosis
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Computer Science Applications
  • Organic Chemistry

これを引用

Development of a fluorescence peptide chip for the detection of caspase activity. / Han, Aishan; Sonoda, Tatsuhiko; Kang, Jeong Hun; Murata, Masaharu; Niiidome, Takuro; Katayama, Yoshiki.

:: Combinatorial Chemistry and High Throughput Screening, 巻 9, 番号 1, 01.01.2006, p. 21-25.

研究成果: ジャーナルへの寄稿記事

Han, Aishan ; Sonoda, Tatsuhiko ; Kang, Jeong Hun ; Murata, Masaharu ; Niiidome, Takuro ; Katayama, Yoshiki. / Development of a fluorescence peptide chip for the detection of caspase activity. :: Combinatorial Chemistry and High Throughput Screening. 2006 ; 巻 9, 番号 1. pp. 21-25.
@article{4cf483652ae2475c91d76e4a63d8f164,
title = "Development of a fluorescence peptide chip for the detection of caspase activity",
abstract = "Proteases play a key role in cell functions, and it is very important to monitor their activities for drug screening and diagnosis of diseases. In the present study, a new class of fluorescence probe, into which a fluorophore and a quencher have been introduced, was developed and applied to the on-chip detection of caspase-3 activity. This probe is non fluorescent in the absence of caspase-3. However, when it is treated with active caspase-3, the fluorescence intensity increases dependent on the caspase-3 activity due to the cleavage of the quencher-containing moiety on a glass slide. This caspase-dependent increase in the fluorescence intensity was also detected when the glass slide immobilizing the probe peptide was treated with cell lysate stimulated by staurosporine (STP), which is an apoptosis-inducing agent. On the other hand, such an increase was not detected in the case of control cell lysate without STP-stimulation. The developed system is a rapid and sensitive method and is useful for the direct measurement of protease activity on a glass array.",
author = "Aishan Han and Tatsuhiko Sonoda and Kang, {Jeong Hun} and Masaharu Murata and Takuro Niiidome and Yoshiki Katayama",
year = "2006",
month = "1",
day = "1",
doi = "10.2174/138620706775213831",
language = "English",
volume = "9",
pages = "21--25",
journal = "Combinatorial Chemistry and High Throughput Screening",
issn = "1386-2073",
publisher = "Bentham Science Publishers B.V.",
number = "1",

}

TY - JOUR

T1 - Development of a fluorescence peptide chip for the detection of caspase activity

AU - Han, Aishan

AU - Sonoda, Tatsuhiko

AU - Kang, Jeong Hun

AU - Murata, Masaharu

AU - Niiidome, Takuro

AU - Katayama, Yoshiki

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Proteases play a key role in cell functions, and it is very important to monitor their activities for drug screening and diagnosis of diseases. In the present study, a new class of fluorescence probe, into which a fluorophore and a quencher have been introduced, was developed and applied to the on-chip detection of caspase-3 activity. This probe is non fluorescent in the absence of caspase-3. However, when it is treated with active caspase-3, the fluorescence intensity increases dependent on the caspase-3 activity due to the cleavage of the quencher-containing moiety on a glass slide. This caspase-dependent increase in the fluorescence intensity was also detected when the glass slide immobilizing the probe peptide was treated with cell lysate stimulated by staurosporine (STP), which is an apoptosis-inducing agent. On the other hand, such an increase was not detected in the case of control cell lysate without STP-stimulation. The developed system is a rapid and sensitive method and is useful for the direct measurement of protease activity on a glass array.

AB - Proteases play a key role in cell functions, and it is very important to monitor their activities for drug screening and diagnosis of diseases. In the present study, a new class of fluorescence probe, into which a fluorophore and a quencher have been introduced, was developed and applied to the on-chip detection of caspase-3 activity. This probe is non fluorescent in the absence of caspase-3. However, when it is treated with active caspase-3, the fluorescence intensity increases dependent on the caspase-3 activity due to the cleavage of the quencher-containing moiety on a glass slide. This caspase-dependent increase in the fluorescence intensity was also detected when the glass slide immobilizing the probe peptide was treated with cell lysate stimulated by staurosporine (STP), which is an apoptosis-inducing agent. On the other hand, such an increase was not detected in the case of control cell lysate without STP-stimulation. The developed system is a rapid and sensitive method and is useful for the direct measurement of protease activity on a glass array.

UR - http://www.scopus.com/inward/record.url?scp=33645751087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645751087&partnerID=8YFLogxK

U2 - 10.2174/138620706775213831

DO - 10.2174/138620706775213831

M3 - Article

C2 - 16454683

AN - SCOPUS:33645751087

VL - 9

SP - 21

EP - 25

JO - Combinatorial Chemistry and High Throughput Screening

JF - Combinatorial Chemistry and High Throughput Screening

SN - 1386-2073

IS - 1

ER -