Development of a fluorescence peptide chip for the detection of caspase activity

Aishan Han, Tatsuhiko Sonoda, Jeong Hun Kang, Masaharu Murata, Takuro Niiidome, Yoshiki Katayama

    研究成果: ジャーナルへの寄稿学術誌査読

    8 被引用数 (Scopus)

    抄録

    Proteases play a key role in cell functions, and it is very important to monitor their activities for drug screening and diagnosis of diseases. In the present study, a new class of fluorescence probe, into which a fluorophore and a quencher have been introduced, was developed and applied to the on-chip detection of caspase-3 activity. This probe is non fluorescent in the absence of caspase-3. However, when it is treated with active caspase-3, the fluorescence intensity increases dependent on the caspase-3 activity due to the cleavage of the quencher-containing moiety on a glass slide. This caspase-dependent increase in the fluorescence intensity was also detected when the glass slide immobilizing the probe peptide was treated with cell lysate stimulated by staurosporine (STP), which is an apoptosis-inducing agent. On the other hand, such an increase was not detected in the case of control cell lysate without STP-stimulation. The developed system is a rapid and sensitive method and is useful for the direct measurement of protease activity on a glass array.

    本文言語英語
    ページ(範囲)21-25
    ページ数5
    ジャーナルCombinatorial Chemistry and High Throughput Screening
    9
    1
    DOI
    出版ステータス出版済み - 1月 1 2006

    !!!All Science Journal Classification (ASJC) codes

    • 創薬
    • コンピュータ サイエンスの応用
    • 有機化学

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