抄録
Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1. °C per min) until -80. °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7. ±. 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3. ±. 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4. ±. 21.6% vs 22.9. ±. 9.3%).
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 283-287 |
| ページ数 | 5 |
| ジャーナル | Cryobiology |
| 巻 | 66 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | 出版済み - 6 1 2013 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
これを引用
Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries. / Banno, Yutaka; Nagasaki, Kiyomi; Tsukada, Marino; Minohara, Yuko; Banno, Junko; Nishikawa, Kazuhiro; Yamamoto, Kazunori; Tamura, Kei; Fujii, Tsuguru.
：: Cryobiology, 巻 66, 番号 3, 01.06.2013, p. 283-287.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries
AU - Banno, Yutaka
AU - Nagasaki, Kiyomi
AU - Tsukada, Marino
AU - Minohara, Yuko
AU - Banno, Junko
AU - Nishikawa, Kazuhiro
AU - Yamamoto, Kazunori
AU - Tamura, Kei
AU - Fujii, Tsuguru
PY - 2013/6/1
Y1 - 2013/6/1
N2 - Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1. °C per min) until -80. °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7. ±. 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3. ±. 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4. ±. 21.6% vs 22.9. ±. 9.3%).
AB - Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1. °C per min) until -80. °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7. ±. 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3. ±. 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4. ±. 21.6% vs 22.9. ±. 9.3%).
UR - http://www.scopus.com/inward/record.url?scp=84877586636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877586636&partnerID=8YFLogxK
U2 - 10.1016/j.cryobiol.2013.03.004
DO - 10.1016/j.cryobiol.2013.03.004
M3 - Article
VL - 66
SP - 283
EP - 287
JO - Cryobiology
JF - Cryobiology
SN - 0011-2240
IS - 3
ER -