Development of a new host vector system in mycobacteria

Yoshitaka Goto, Hatsumi Taniguchi, Takezo Udou, Yasuo Mizuguchi, Tohru Tokunaga

研究成果: Contribution to journalArticle

18 引用 (Scopus)

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The hybrid plasmid pYT72/pYT92 constructed from an Escherichia coli plasmid pACYC177 and mycobacterial plasmid pMSC262 isolated from Mycobacterium scroflaceum strain W262 transformed both E. coli and BCG. Phage-sensitive mutants S-10 and S-20 isolated from BCG Tokyo strain showed higher frequency of transformation than the wild-type strain. Frequency of transformation was dependent on age of the culture and the electroporation condition used. Several deletion mutants were generated from pYT72/92 to determine the minimum region for the replication in the mycobacteria. A 2.3-kb fragment of pMSC262 was found to contain an essential region. Using this fragment and pACYC177, a small shuttle vector pYT937 containing two drug-resistance markers, kanamycin- and ampicillin-resistance, was constructed. pYT937 contains AatII, BamHI, BbvII, GsuI, HincII, PstI, ScaI and XbaI cloning sites.

元の言語英語
ページ(範囲)277-282
ページ数6
ジャーナルFEMS microbiology letters
83
発行部数3
DOI
出版物ステータス出版済み - 10 15 1991
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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  • これを引用

    Goto, Y., Taniguchi, H., Udou, T., Mizuguchi, Y., & Tokunaga, T. (1991). Development of a new host vector system in mycobacteria. FEMS microbiology letters, 83(3), 277-282. https://doi.org/10.1111/j.1574-6968.1991.tb04477.x