Development of a new measurement method for serum calcium with chlorophosphonazo-III

Eisaku Hokazono, Susumu Osawa, Tomota Nakano, Yukari Kawamoto, Yuji Oguchi, Taeko Hotta, Yuzo Kayamori, Dongchon Kang, Yuichiro Cho, Kiyoko Shiba, Kenji Sato

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Background: Although serum calcium has been measured using the o-cresolphthalein complexone (oCPC) method in the clinical laboratory, this method still has some problems regarding linearity and reagent stability. We developed a new measurement procedure using chlorophosphonazo-III (CPZ-III: 2,7-bis (4-chloro-2-phosphonophenylazo) -1,8- dihydroxy-3, 6- naphthalenedisulphonic acid, disodium salt) as a chelator with an acid medium for serum calcium measurement. The present method showed better linearity and reagent stability compared with the oCPC method. Methods: Characteristics were studied in optimized conditions measuring wavelength by absorption spectra analysis, and interference of protein and metals with Mg2+, Fe 2+, Cu2+ and Zn2+. The method was applied to an automated analyser (7170; Hitachi High Technologies Corp). The measurement performance was evaluated for accuracy, precision, recovery rate, linearity and reagent stability with a comparison study against atomic absorption spectrophotometry (AAS). Results: The within-run and between-run variations (coefficient of variation [CV]) were 0.92-1.01% and 0.75-1.43%, respectively. The linearity was 0-7.0 mmol/L. The comparison study obtained y = 1.002x (AAS) - 0.10, Sy/x = 0.18 mmol/L, n = 50. Reagent stability was at least 20 d at 48C without daily calibration. Conclusion: The new calcium measurement method in serum was demonstrated to have reliable and acceptable performances as a routine test in clinical laboratory.

元の言語英語
ページ(範囲)296-301
ページ数6
ジャーナルAnnals of Clinical Biochemistry
46
発行部数4
DOI
出版物ステータス出版済み - 7 1 2009

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Clinical laboratories
Calcium
Spectrophotometry
Serum
Wave interference
Atomic Spectrophotometry
Acids
Chelating Agents
Spectrum analysis
Absorption spectra
Salts
Metals
Calibration
Recovery
Wavelength
Spectrum Analysis
Proteins
Technology
2-cresolphthalexon

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

これを引用

Development of a new measurement method for serum calcium with chlorophosphonazo-III. / Hokazono, Eisaku; Osawa, Susumu; Nakano, Tomota; Kawamoto, Yukari; Oguchi, Yuji; Hotta, Taeko; Kayamori, Yuzo; Kang, Dongchon; Cho, Yuichiro; Shiba, Kiyoko; Sato, Kenji.

:: Annals of Clinical Biochemistry, 巻 46, 番号 4, 01.07.2009, p. 296-301.

研究成果: ジャーナルへの寄稿記事

Hokazono, E, Osawa, S, Nakano, T, Kawamoto, Y, Oguchi, Y, Hotta, T, Kayamori, Y, Kang, D, Cho, Y, Shiba, K & Sato, K 2009, 'Development of a new measurement method for serum calcium with chlorophosphonazo-III', Annals of Clinical Biochemistry, 巻. 46, 番号 4, pp. 296-301. https://doi.org/10.1258/acb.2009.008099
Hokazono, Eisaku ; Osawa, Susumu ; Nakano, Tomota ; Kawamoto, Yukari ; Oguchi, Yuji ; Hotta, Taeko ; Kayamori, Yuzo ; Kang, Dongchon ; Cho, Yuichiro ; Shiba, Kiyoko ; Sato, Kenji. / Development of a new measurement method for serum calcium with chlorophosphonazo-III. :: Annals of Clinical Biochemistry. 2009 ; 巻 46, 番号 4. pp. 296-301.
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abstract = "Background: Although serum calcium has been measured using the o-cresolphthalein complexone (oCPC) method in the clinical laboratory, this method still has some problems regarding linearity and reagent stability. We developed a new measurement procedure using chlorophosphonazo-III (CPZ-III: 2,7-bis (4-chloro-2-phosphonophenylazo) -1,8- dihydroxy-3, 6- naphthalenedisulphonic acid, disodium salt) as a chelator with an acid medium for serum calcium measurement. The present method showed better linearity and reagent stability compared with the oCPC method. Methods: Characteristics were studied in optimized conditions measuring wavelength by absorption spectra analysis, and interference of protein and metals with Mg2+, Fe 2+, Cu2+ and Zn2+. The method was applied to an automated analyser (7170; Hitachi High Technologies Corp). The measurement performance was evaluated for accuracy, precision, recovery rate, linearity and reagent stability with a comparison study against atomic absorption spectrophotometry (AAS). Results: The within-run and between-run variations (coefficient of variation [CV]) were 0.92-1.01{\%} and 0.75-1.43{\%}, respectively. The linearity was 0-7.0 mmol/L. The comparison study obtained y = 1.002x (AAS) - 0.10, Sy/x = 0.18 mmol/L, n = 50. Reagent stability was at least 20 d at 48C without daily calibration. Conclusion: The new calcium measurement method in serum was demonstrated to have reliable and acceptable performances as a routine test in clinical laboratory.",
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AU - Hokazono, Eisaku

AU - Osawa, Susumu

AU - Nakano, Tomota

AU - Kawamoto, Yukari

AU - Oguchi, Yuji

AU - Hotta, Taeko

AU - Kayamori, Yuzo

AU - Kang, Dongchon

AU - Cho, Yuichiro

AU - Shiba, Kiyoko

AU - Sato, Kenji

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N2 - Background: Although serum calcium has been measured using the o-cresolphthalein complexone (oCPC) method in the clinical laboratory, this method still has some problems regarding linearity and reagent stability. We developed a new measurement procedure using chlorophosphonazo-III (CPZ-III: 2,7-bis (4-chloro-2-phosphonophenylazo) -1,8- dihydroxy-3, 6- naphthalenedisulphonic acid, disodium salt) as a chelator with an acid medium for serum calcium measurement. The present method showed better linearity and reagent stability compared with the oCPC method. Methods: Characteristics were studied in optimized conditions measuring wavelength by absorption spectra analysis, and interference of protein and metals with Mg2+, Fe 2+, Cu2+ and Zn2+. The method was applied to an automated analyser (7170; Hitachi High Technologies Corp). The measurement performance was evaluated for accuracy, precision, recovery rate, linearity and reagent stability with a comparison study against atomic absorption spectrophotometry (AAS). Results: The within-run and between-run variations (coefficient of variation [CV]) were 0.92-1.01% and 0.75-1.43%, respectively. The linearity was 0-7.0 mmol/L. The comparison study obtained y = 1.002x (AAS) - 0.10, Sy/x = 0.18 mmol/L, n = 50. Reagent stability was at least 20 d at 48C without daily calibration. Conclusion: The new calcium measurement method in serum was demonstrated to have reliable and acceptable performances as a routine test in clinical laboratory.

AB - Background: Although serum calcium has been measured using the o-cresolphthalein complexone (oCPC) method in the clinical laboratory, this method still has some problems regarding linearity and reagent stability. We developed a new measurement procedure using chlorophosphonazo-III (CPZ-III: 2,7-bis (4-chloro-2-phosphonophenylazo) -1,8- dihydroxy-3, 6- naphthalenedisulphonic acid, disodium salt) as a chelator with an acid medium for serum calcium measurement. The present method showed better linearity and reagent stability compared with the oCPC method. Methods: Characteristics were studied in optimized conditions measuring wavelength by absorption spectra analysis, and interference of protein and metals with Mg2+, Fe 2+, Cu2+ and Zn2+. The method was applied to an automated analyser (7170; Hitachi High Technologies Corp). The measurement performance was evaluated for accuracy, precision, recovery rate, linearity and reagent stability with a comparison study against atomic absorption spectrophotometry (AAS). Results: The within-run and between-run variations (coefficient of variation [CV]) were 0.92-1.01% and 0.75-1.43%, respectively. The linearity was 0-7.0 mmol/L. The comparison study obtained y = 1.002x (AAS) - 0.10, Sy/x = 0.18 mmol/L, n = 50. Reagent stability was at least 20 d at 48C without daily calibration. Conclusion: The new calcium measurement method in serum was demonstrated to have reliable and acceptable performances as a routine test in clinical laboratory.

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