Development of a novel polycationic adsorbent for cryogel removal

Keiichi Miyamoto, Ryuji Uchiyama, Masayuki Tokita, Motoki Yonekawa, Akio Kawamura, Keiichi Miyashita, Eiji Sakashita, Takashi Komai

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

Cryogel, prevalent in the plasma of rheumatism patients, is a plasma fibronectin (pFN)-extra domain A containing FN (EDA(+)FN)-fibrinogen (Fbg) complex formed by adding heparin (HP) at a low temperature (4°C). Although EDA(+)FN does not usually exist in normal plasma, its prevalence in rheumatic patients causes cryogelation in plasma. Removal of cryogel is thus a promising and novel approach to treating rheumatism. As HP-EDA(+)FN aggregate, which is induced by the main component of cryogel, is considered to be an anion, cationic materials capable of eliminating this anionic conjugate were innovated in this study. We found that an amino group density of 100-130 μmol/g (dry weight) of adsorbents prompted selective adsorption of the EDA(+)FN-HP complex. Elimination of EDA(+)FN as high as 80% accompanied by removal of the components of total FN (pFN) (10%) and Fbg (10%) in the model patient plasma was established.

本文言語英語
ページ(範囲)19-24
ページ数6
ジャーナルInternational Journal of Biological Macromolecules
29
1
DOI
出版ステータス出版済み - 7月 19 2001
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生化学
  • 分子生物学
  • 経済学、計量経済学
  • エネルギー(全般)

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