ブレビバチルス発現系によるペルオキシダーゼ融合タンパク質試薬の調製

林 浩之輔, 友添 祐介, 永井 賢治, 平石 佳之, 神谷 典穂

研究成果: Contribution to journalArticle査読

抄録

To detect a target protein in biological samples, a fusion protein was designed composed of a peroxidase from <i>Arthromyces ramosus</i> (ARP) and parts of the antibody-binding domains of <i>Staphylococcus aureus</i> protein A and <i>Streptococcus</i> protein G (PG). The ARP-PG fusion protein was successfully expressed by a heterologous protein expression system in <i>Brevibacillus choshinensis</i>. The fusion protein was secreted as an active form in culture media. The production of ARP-PG with higher peroxidase activity was observed by the addition of 5-aminolevulinic acid to the culture media. The performance of purified ARP-PG was validated by dot blotting for the detection of transferrin as a model target protein. A comparable performance in the dot blot analysis was attained using a culture supernatant containing crude but active ARP-PG, indicating the practicality of the <i>Brevibacillus</i> protein secretion system.
寄稿の翻訳タイトルDevelopment of a Peroxidase-Fused Protein Reagent by <i>Brevibacillus choshinensis</i> Heterologous Expression System
本文言語Japanese
ページ(範囲)157-161
ページ数5
ジャーナルKagaku Kogaku Ronbunshu
41
2
DOI
出版ステータス出版済み - 2015

フィンガープリント 「ブレビバチルス発現系によるペルオキシダーゼ融合タンパク質試薬の調製」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル