Development of a sample preparation method for fungal proteomics

Motoyuki Shimizu, Hiroyuki Wariishi

研究成果: ジャーナルへの寄稿学術誌査読

28 被引用数 (Scopus)


Since filamentous fungi including basidiomycetous fungi possess an exceptionally robust cell wall as in microorganisms, effective extraction of intracellular proteins is a key step for fungal proteomic studies. To overcome the experimental obstacle caused by cell walls, we utilized fungal protoplasts, prepared from the brown-rot basidiomycete, Tyromyces palustris. The amount and quality of proteins extracted from the protoplast cells were much higher than that from the mycelial cells. Quantitative comparisons of proteome maps prepared from mycelial and protoplast cells indicated protein spots with a wider range of molecular weights and pIs in the protoplast sample. Furthermore, no streaking or tailing was observed in the protoplasts, suggesting that effective extraction of intracellular proteins from protoplasts might help suppress degradation of proteins during this process. In addition to the efficiency of protein extraction, simple and efficient subcellular fractionation was also achieved using protoplast cells.

ジャーナルFEMS microbiology letters
出版ステータス出版済み - 6月 1 2005

!!!All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学
  • 遺伝学


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