Development of a sensitive ELISA for human leptin, using monoclonal antibodies

Keiichi Imagawa, Yayoi Matsumoto, Yoshito Numata, Atsushi Morita, Shino Kikuoka, Mikio Tamaki, Chie Higashikubo, Tetsuo Tsuji, Kazuyuki Sasakura, Hiroshi Teraoka, Hiroaki Masuzaki, Kiminori Hosoda, Yoshihiro Ogawa, Kazuwa Nakao

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

A new, sensitive ELISA for human leptin in plasma and cerebrospinal fluid (CSF) was developed, using monoclonal antibodies. The lower limit of detection of this ELISA was 0.78 pg/assay. Both intra- and interassay imprecision values were <7%. The dilution curves of plasma and CSF showed good linearity, and the recovery was 83.2-95.6%. There was good correlation between plasma leptin concentrations by the ELISA and a commercially available RIA (r = 0.99). Our ELISA is advantageous because it does not require radioisotopes, it produces results in hours rather than days, and more importantly, it improves on the detection limit and plasma interference of the RIA kit. The new ELISA enables measurement of low concentrations of leptin, as are seen in CSF and in plasma of patients with anorexia nervosa.

元の言語英語
ページ(範囲)2165-2171
ページ数7
ジャーナルClinical Chemistry
44
発行部数10
出版物ステータス出版済み - 10 12 1998
外部発表Yes

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Leptin
Cerebrospinal fluid
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Plasmas
Cerebrospinal Fluid
Limit of Detection
Anorexia Nervosa
Radioisotopes
Dilution
Assays
Recovery

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

これを引用

Imagawa, K., Matsumoto, Y., Numata, Y., Morita, A., Kikuoka, S., Tamaki, M., ... Nakao, K. (1998). Development of a sensitive ELISA for human leptin, using monoclonal antibodies. Clinical Chemistry, 44(10), 2165-2171.

Development of a sensitive ELISA for human leptin, using monoclonal antibodies. / Imagawa, Keiichi; Matsumoto, Yayoi; Numata, Yoshito; Morita, Atsushi; Kikuoka, Shino; Tamaki, Mikio; Higashikubo, Chie; Tsuji, Tetsuo; Sasakura, Kazuyuki; Teraoka, Hiroshi; Masuzaki, Hiroaki; Hosoda, Kiminori; Ogawa, Yoshihiro; Nakao, Kazuwa.

:: Clinical Chemistry, 巻 44, 番号 10, 12.10.1998, p. 2165-2171.

研究成果: ジャーナルへの寄稿記事

Imagawa, K, Matsumoto, Y, Numata, Y, Morita, A, Kikuoka, S, Tamaki, M, Higashikubo, C, Tsuji, T, Sasakura, K, Teraoka, H, Masuzaki, H, Hosoda, K, Ogawa, Y & Nakao, K 1998, 'Development of a sensitive ELISA for human leptin, using monoclonal antibodies', Clinical Chemistry, 巻. 44, 番号 10, pp. 2165-2171.
Imagawa K, Matsumoto Y, Numata Y, Morita A, Kikuoka S, Tamaki M その他. Development of a sensitive ELISA for human leptin, using monoclonal antibodies. Clinical Chemistry. 1998 10 12;44(10):2165-2171.
Imagawa, Keiichi ; Matsumoto, Yayoi ; Numata, Yoshito ; Morita, Atsushi ; Kikuoka, Shino ; Tamaki, Mikio ; Higashikubo, Chie ; Tsuji, Tetsuo ; Sasakura, Kazuyuki ; Teraoka, Hiroshi ; Masuzaki, Hiroaki ; Hosoda, Kiminori ; Ogawa, Yoshihiro ; Nakao, Kazuwa. / Development of a sensitive ELISA for human leptin, using monoclonal antibodies. :: Clinical Chemistry. 1998 ; 巻 44, 番号 10. pp. 2165-2171.
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