Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test

Jiranan Chaingam, Tharita Kitisripanya, Supaluk Krittanai, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun

研究成果: ジャーナルへの寄稿記事

抄録

Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.

元の言語英語
ページ(範囲)577-583
ページ数7
ジャーナルJournal of Natural Medicines
73
発行部数3
DOI
出版物ステータス出版済み - 6 14 2019

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Pueraria
Standardization
Benzopyrans
Gold Colloid
Immunosorbents
Assays
Estrogens
Monoclonal Antibodies
Nanoparticles
Enzymes
Estrogen Replacement Therapy
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

これを引用

Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test. / Chaingam, Jiranan; Kitisripanya, Tharita; Krittanai, Supaluk; Sakamoto, Seiichi; Tanaka, Hiroyuki; Putalun, Waraporn.

:: Journal of Natural Medicines, 巻 73, 番号 3, 14.06.2019, p. 577-583.

研究成果: ジャーナルへの寄稿記事

Chaingam, Jiranan ; Kitisripanya, Tharita ; Krittanai, Supaluk ; Sakamoto, Seiichi ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test. :: Journal of Natural Medicines. 2019 ; 巻 73, 番号 3. pp. 577-583.
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abstract = "Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.",
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