Development of an anti-norovirus single-chain Fv for immunochromatographic test kit.

Michihiko Nakano, Keita Murofushi, Satoshi Watabe, Toshiya Ohta

研究成果: ジャーナルへの寄稿記事

抄録

It was developed that an anti-norovirus single-chain Fv (scFv) antibody for immunochromathographic test kit by using the phage displayed technique and biopanning. To obtain the scFv having wide reactivity for several norovirus genotypes, a mixture of recombinant norovirus capsid proteins, including 11 norovirus genotypes, was used for biopanning. Then, one anti-norovirus scFv antibody that recognized both of norovirus genogroups GI and GII was identified. An immunochromatographic test strip was constructed by using the scFv and demonstrated it to detect norovirus in stool samples. The immunochromatographic strip showed similar sensitivity to a commercially available one on which several monoclonal antibodies are included.

元の言語英語
ページ(範囲)69-73
ページ数5
ジャーナルUnknown Journal
23
発行部数2
出版物ステータス出版済み - 2013

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Norovirus
Single-Chain Antibodies
Genotype
Capsid Proteins
Bacteriophages
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Medicine(all)

これを引用

Development of an anti-norovirus single-chain Fv for immunochromatographic test kit. / Nakano, Michihiko; Murofushi, Keita; Watabe, Satoshi; Ohta, Toshiya.

:: Unknown Journal, 巻 23, 番号 2, 2013, p. 69-73.

研究成果: ジャーナルへの寄稿記事

Nakano, M, Murofushi, K, Watabe, S & Ohta, T 2013, 'Development of an anti-norovirus single-chain Fv for immunochromatographic test kit.', Unknown Journal, 巻. 23, 番号 2, pp. 69-73.
Nakano, Michihiko ; Murofushi, Keita ; Watabe, Satoshi ; Ohta, Toshiya. / Development of an anti-norovirus single-chain Fv for immunochromatographic test kit. :: Unknown Journal. 2013 ; 巻 23, 番号 2. pp. 69-73.
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