Development of an anti-norovirus single-chain Fv for immunochromatographic test kit.

Michihiko Nakano, Keita Murofushi, Satoshi Watabe, Toshiya Ohta

研究成果: Contribution to journalArticle査読

抄録

It was developed that an anti-norovirus single-chain Fv (scFv) antibody for immunochromathographic test kit by using the phage displayed technique and biopanning. To obtain the scFv having wide reactivity for several norovirus genotypes, a mixture of recombinant norovirus capsid proteins, including 11 norovirus genotypes, was used for biopanning. Then, one anti-norovirus scFv antibody that recognized both of norovirus genogroups GI and GII was identified. An immunochromatographic test strip was constructed by using the scFv and demonstrated it to detect norovirus in stool samples. The immunochromatographic strip showed similar sensitivity to a commercially available one on which several monoclonal antibodies are included.

本文言語英語
ページ(範囲)69-73
ページ数5
ジャーナルUnknown Journal
23
2
出版ステータス出版済み - 2013

All Science Journal Classification (ASJC) codes

  • 医学(全般)

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