Development of an artificial cell, from self-organization to computation and self-reproduction

Vincent Noireaux, Yusuke Maeda, Albert Libchaber

研究成果: ジャーナルへの寄稿評論記事

167 引用 (Scopus)

抄録

This article describes the state and the development of an artificial cell project. We discuss the experimental constraints to synthesize the most elementary cell-sized compartment that can self-reproduce using synthetic genetic information. The original ideawas to program a phospholipid vesicle with DNA. Based on this idea, it was shown that in vitro gene expression could be carried out inside cell-sized synthetic vesicles. It was also shown that a couple of genes could be expressed for a few days inside the vesicles once the exchanges of nutrients with the outside environment were adequately introduced. The development of a cell-free transcription/translation toolbox allows the expression of a large number of genes with multiple transcription factors. As a result, the development of a synthetic DNA program is becoming one of themain hurdles. We discuss the various possibilities to enrich and to replicate this program. Defining a program for self-reproduction remains a difficult question as nongenetic processes, such as molecular self-organization, play an essential and complementary role. The synthesis of a stable compartment with an active interface, one of the critical bottlenecks in the synthesis of artificial cell, depends on the properties of phospholipid membranes. The problem of a self-replicating artificial cell is a long-lasting goal that might imply evolution experiments.

元の言語英語
ページ(範囲)3473-3480
ページ数8
ジャーナルProceedings of the National Academy of Sciences of the United States of America
108
発行部数9
DOI
出版物ステータス出版済み - 3 1 2011

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Artificial Cells
Reproduction
Phospholipids
DNA
Genes
Transcription Factors
Gene Expression
Food
Membranes

All Science Journal Classification (ASJC) codes

  • General

これを引用

Development of an artificial cell, from self-organization to computation and self-reproduction. / Noireaux, Vincent; Maeda, Yusuke; Libchaber, Albert.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 108, 番号 9, 01.03.2011, p. 3473-3480.

研究成果: ジャーナルへの寄稿評論記事

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