Development of an easy and high-throughput cell assay system with a culture chip and an assay chip

Kanako Sugiura, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

High throughput cell assay is significantly important in drug screening, assessment of toxicity etc. Cell assay with a microchip is one of the candidates for high throughput cell assay. However, reported cell assay system with the microchip requires expensive apparatus for refluxing medium and investigation of optimum experimental condition for steady data. For an inexpensive, easy and high throughput cell assay, we introduce a new cell assay system combined with a culture chip and an assay chip made of poly(dimethyl siloxane). Cell culture chips enabled cell to proliferate along the microchannel without refluxing medium and permitted to prepare cell patterning easily. Also, assay chips formed concentration gradient inside the chip and allowed the cell assay with different concentrations of drug at the same time. Thus, our developed cell assay system can overcome the problems of the present cell assay and would promote the drug discovery, assessment of toxicity etc.

本文言語英語
ページ(範囲)471-475
ページ数5
ジャーナルIEEJ Transactions on Sensors and Micromachines
130
10
DOI
出版ステータス出版済み - 2010
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 機械工学
  • 電子工学および電気工学

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