Development of an enzyme immunoassay using a monoclonal antibody against the psychoactive diterpenoid salvinorin A

Madan Kumar Paudel, Osamu Shirota, Kaori Tabata, Hiroyuki Tanaka, Setsuko Sekita, Satoshi Morimoto

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Salvinorin A (1), the main active constituent in Salvia divinorum, is a highly selective kappa-opioid receptor agonist with hallucinogenic effects, which is regulated in several countries. In the present study, a monoclonal antibody (mAb) against 1 was prepared, and an indirect competitive enzyme-linked immunosorbent assay (icELISA) system was developed for the detection of salvinorins. To raise mAbs against 1, salvinorin B (2) hemisuccinate was synthesized and used to prepare the immunogen 2-bovine serum albumin conjugate. This technique was used to prepare a hybridoma cell line, 3D5, which secreted a mAb that recognized 1. The mAb was shown to have specificity for 1 and other salvinorins in cross-reactivity tests. The intra-assay calibration range by icELISA using the mAb against 1 was 0.0195-0.625 μg/mL. After validating the icELISA using intra- and interassays, a recovery experiment and analysis of several plants in the family Lamiaceae, including S. divinorum, confirmed that the analytical method based on ELISA is not only simple but also precise, accurate, sensitive, and sufficiently reliable. The results indicate that icELISA is a useful tool in the identification of S. divinorum.

元の言語英語
ページ(範囲)1654-1660
ページ数7
ジャーナルJournal of Natural Products
76
発行部数9
DOI
出版物ステータス出版済み - 9 27 2013

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salvinorin A
Diterpenes
Immunosorbents
Immunoenzyme Techniques
Assays
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Enzymes
Salvia
Lamiaceae
kappa Opioid Receptor
Hybridomas
Bovine Serum Albumin
Calibration
Cells
Recovery
Cell Line

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

これを引用

Development of an enzyme immunoassay using a monoclonal antibody against the psychoactive diterpenoid salvinorin A. / Paudel, Madan Kumar; Shirota, Osamu; Tabata, Kaori; Tanaka, Hiroyuki; Sekita, Setsuko; Morimoto, Satoshi.

:: Journal of Natural Products, 巻 76, 番号 9, 27.09.2013, p. 1654-1660.

研究成果: ジャーナルへの寄稿記事

Paudel, Madan Kumar ; Shirota, Osamu ; Tabata, Kaori ; Tanaka, Hiroyuki ; Sekita, Setsuko ; Morimoto, Satoshi. / Development of an enzyme immunoassay using a monoclonal antibody against the psychoactive diterpenoid salvinorin A. :: Journal of Natural Products. 2013 ; 巻 76, 番号 9. pp. 1654-1660.
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