Development of an in vitro model for the analysis of bovine endometrium using simple techniques

Keisuke Yamauchi, Nobuhiko Yamauchi, Kazuki Yamagami, Nobuhisa Nakamura, Seiya Yamashita, Md Rashedul Islam, Shoji Tabata, Kanji Yahiro, Tomoko Tamura, Kazuyoshi Hashizume, Masa aki Hattori

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

This study aimed to develop an in vitro model for the analysis of the bovine endometrium. Immunofluorescent staining revealed that the hetero-spheroids and the cultured explants showed almost similar structure in the localization of bovine endometrial epithelial cells and endometrial stromal cells, except the glandular-like structure of the epithelial cells inside the explants. Gelatin zymography revealed that the hetero-spheroids did not express matrix metalloproteinases (MMPs) after 4 days of culture, but strong MMP expressions were observed in the cultured explants until 7 days of culture. Additionally, expression of progesterone receptor (PR), estrogen receptor alpha (ERα), type I interferon receptor 1 (IFNAR1) and 2 (IFNAR2) messenger RNA was observed both in the homo- and hetero-spheroids. The expression of oxytocin receptor (OTR) mRNA in E2 and E2+P4 (1,3,5(10)-Estratrien-3, 17β-diol + 4-Pregnen-3, 20-dinone) treated groups were significantly (P<0.05) higher than that of the control group of spheroids. In case of cultured explants, the expression of PR and OTR mRNA were significantly (P<0.05) higher in E2 treated groups compared to the control groups. Hepatocyte growth factor (HGF) mRNA expression was also higher in P4 treated groups at 10 days in culture (P<0.05). In a nutshell, the in vitro model developed in this study for the analysis of the endometrium may provide a new platform for extensive research on bovine endometrial function.

元の言語英語
ページ(範囲)523-531
ページ数9
ジャーナルAnimal Science Journal
86
発行部数5
DOI
出版物ステータス出版済み - 5 1 2015

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endometrium
Endometrium
explants
Oxytocin Receptors
Messenger RNA
cattle
metalloproteinases
Progesterone Receptors
Matrix Metalloproteinases
epithelial cells
Epithelial Cells
Interferon alpha-beta Receptor
hepatocyte growth factor
Control Groups
glycols
Hepatocyte Growth Factor
Estrogen Receptor alpha
stromal cells
Homo
interferons

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

これを引用

Yamauchi, K., Yamauchi, N., Yamagami, K., Nakamura, N., Yamashita, S., Islam, M. R., ... Hattori, M. A. (2015). Development of an in vitro model for the analysis of bovine endometrium using simple techniques. Animal Science Journal, 86(5), 523-531. https://doi.org/10.1111/asj.12318

Development of an in vitro model for the analysis of bovine endometrium using simple techniques. / Yamauchi, Keisuke; Yamauchi, Nobuhiko; Yamagami, Kazuki; Nakamura, Nobuhisa; Yamashita, Seiya; Islam, Md Rashedul; Tabata, Shoji; Yahiro, Kanji; Tamura, Tomoko; Hashizume, Kazuyoshi; Hattori, Masa aki.

:: Animal Science Journal, 巻 86, 番号 5, 01.05.2015, p. 523-531.

研究成果: ジャーナルへの寄稿記事

Yamauchi, K, Yamauchi, N, Yamagami, K, Nakamura, N, Yamashita, S, Islam, MR, Tabata, S, Yahiro, K, Tamura, T, Hashizume, K & Hattori, MA 2015, 'Development of an in vitro model for the analysis of bovine endometrium using simple techniques', Animal Science Journal, 巻. 86, 番号 5, pp. 523-531. https://doi.org/10.1111/asj.12318
Yamauchi, Keisuke ; Yamauchi, Nobuhiko ; Yamagami, Kazuki ; Nakamura, Nobuhisa ; Yamashita, Seiya ; Islam, Md Rashedul ; Tabata, Shoji ; Yahiro, Kanji ; Tamura, Tomoko ; Hashizume, Kazuyoshi ; Hattori, Masa aki. / Development of an in vitro model for the analysis of bovine endometrium using simple techniques. :: Animal Science Journal. 2015 ; 巻 86, 番号 5. pp. 523-531.
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AU - Yamashita, Seiya

AU - Islam, Md Rashedul

AU - Tabata, Shoji

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