Development of an online two-dimensional high-performance liquid chromatographic system in combination with tandem mass spectrometric detection for enantiomeric analysis of free amino acids in human physiological fluid

Chiharu Ishii, Takeyuki Akita, Masashi Mita, Tomomi Ide, Kenji Hamase

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

An automated two-dimensional HPLC-MS/MS system was designed and developed for the highly selective determination of trace levels of D-amino acids. As the targets, frequently observed ones in mammalian physiological fluids, Ala, Asp, Glu, Leu, Pro and Ser, were selected because these D-amino acids are the potential biomarkers for the early and sensitive diagnoses of various diseases. The target analytes were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), then isolated by a reversed-phase column in the first dimension. The NBD-amino acid fractions were automatically collected into the multi-loop device, and introduced into the enantioselective column in the second dimension to separate the D- and L-forms followed by detection by an MS/MS. The obtained resolution values of the enantiomers were 1.87–5.17 and the calibration lines, precision and accuracy were practically sufficient. By using the present 2D HPLC-MS/MS system, trace levels of D-amino acids in complex biological matrices were determined without disturbance by the intrinsic interfering compounds, and successfully applied to the analysis of the human clinical samples (plasma and urine).

元の言語英語
ページ(範囲)91-98
ページ数8
ジャーナルJournal of Chromatography A
1570
DOI
出版物ステータス出版済み - 10 5 2018

Fingerprint

Amino Acids
Fluids
Liquids
High Pressure Liquid Chromatography
L Forms
Enantiomers
Biomarkers
Calibration
Early Diagnosis
Urine
Plasmas
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

これを引用

@article{655df79aa59f48b7902b99ae242fe695,
title = "Development of an online two-dimensional high-performance liquid chromatographic system in combination with tandem mass spectrometric detection for enantiomeric analysis of free amino acids in human physiological fluid",
abstract = "An automated two-dimensional HPLC-MS/MS system was designed and developed for the highly selective determination of trace levels of D-amino acids. As the targets, frequently observed ones in mammalian physiological fluids, Ala, Asp, Glu, Leu, Pro and Ser, were selected because these D-amino acids are the potential biomarkers for the early and sensitive diagnoses of various diseases. The target analytes were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), then isolated by a reversed-phase column in the first dimension. The NBD-amino acid fractions were automatically collected into the multi-loop device, and introduced into the enantioselective column in the second dimension to separate the D- and L-forms followed by detection by an MS/MS. The obtained resolution values of the enantiomers were 1.87–5.17 and the calibration lines, precision and accuracy were practically sufficient. By using the present 2D HPLC-MS/MS system, trace levels of D-amino acids in complex biological matrices were determined without disturbance by the intrinsic interfering compounds, and successfully applied to the analysis of the human clinical samples (plasma and urine).",
author = "Chiharu Ishii and Takeyuki Akita and Masashi Mita and Tomomi Ide and Kenji Hamase",
year = "2018",
month = "10",
day = "5",
doi = "10.1016/j.chroma.2018.07.076",
language = "English",
volume = "1570",
pages = "91--98",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",

}

TY - JOUR

T1 - Development of an online two-dimensional high-performance liquid chromatographic system in combination with tandem mass spectrometric detection for enantiomeric analysis of free amino acids in human physiological fluid

AU - Ishii, Chiharu

AU - Akita, Takeyuki

AU - Mita, Masashi

AU - Ide, Tomomi

AU - Hamase, Kenji

PY - 2018/10/5

Y1 - 2018/10/5

N2 - An automated two-dimensional HPLC-MS/MS system was designed and developed for the highly selective determination of trace levels of D-amino acids. As the targets, frequently observed ones in mammalian physiological fluids, Ala, Asp, Glu, Leu, Pro and Ser, were selected because these D-amino acids are the potential biomarkers for the early and sensitive diagnoses of various diseases. The target analytes were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), then isolated by a reversed-phase column in the first dimension. The NBD-amino acid fractions were automatically collected into the multi-loop device, and introduced into the enantioselective column in the second dimension to separate the D- and L-forms followed by detection by an MS/MS. The obtained resolution values of the enantiomers were 1.87–5.17 and the calibration lines, precision and accuracy were practically sufficient. By using the present 2D HPLC-MS/MS system, trace levels of D-amino acids in complex biological matrices were determined without disturbance by the intrinsic interfering compounds, and successfully applied to the analysis of the human clinical samples (plasma and urine).

AB - An automated two-dimensional HPLC-MS/MS system was designed and developed for the highly selective determination of trace levels of D-amino acids. As the targets, frequently observed ones in mammalian physiological fluids, Ala, Asp, Glu, Leu, Pro and Ser, were selected because these D-amino acids are the potential biomarkers for the early and sensitive diagnoses of various diseases. The target analytes were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), then isolated by a reversed-phase column in the first dimension. The NBD-amino acid fractions were automatically collected into the multi-loop device, and introduced into the enantioselective column in the second dimension to separate the D- and L-forms followed by detection by an MS/MS. The obtained resolution values of the enantiomers were 1.87–5.17 and the calibration lines, precision and accuracy were practically sufficient. By using the present 2D HPLC-MS/MS system, trace levels of D-amino acids in complex biological matrices were determined without disturbance by the intrinsic interfering compounds, and successfully applied to the analysis of the human clinical samples (plasma and urine).

UR - http://www.scopus.com/inward/record.url?scp=85051264327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051264327&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2018.07.076

DO - 10.1016/j.chroma.2018.07.076

M3 - Article

C2 - 30104059

AN - SCOPUS:85051264327

VL - 1570

SP - 91

EP - 98

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

ER -