@article{a8006ffe7ec44c3fa03a2e40f6bc50b9,
title = "Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of “new ulcer disease⇝ in koi carp",
abstract = "To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromo-nads.",
author = "Tomonori Somamoto and Shinpei Maruyama and Takahiro Nagasawa and Miki Nakao and Atsushi Sato and Hajime Hatta and Mitsuru Sato and Yukie Murakami-Yamaguchi and Kumiko Kizu-Mori and Yuki Hirakawa and Hiroshi Narita",
note = "Funding Information: The authors wish to express their appreciation to Ms. Yuka Murakami for her assistance to this work. This work was partly supported by MEXT Project for Developing Innovation Systems (Regional Innovation Strategy Support Program {\textquoteleft}Kyoto Environmental Nanotechnology Cluster 2008–2013{\textquoteright}). The authors would like to thank Mr. Shoh Sato and Ms. Kazue Nakajima of Niigata Prefectural Inland-water Fisheries Experimental Station for kindly providing isolates of atypical A. salmonicida from carp. The authors would like to thank Enago (www.enago.jp) for the English language review. Publisher Copyright: {\textcopyright} 2018 The Japanese Society of Fish Pathology.",
year = "2018",
doi = "10.3147/jsfp.53.36",
language = "English",
volume = "53",
pages = "36--39",
journal = "Fish Pathology",
issn = "0388-788X",
publisher = "Japanese Society of Fish Pathology",
number = "1",
}