抄録
To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromo-nads.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 36-39 |
| ページ数 | 4 |
| ジャーナル | Fish Pathology |
| 巻 | 53 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2018 |
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All Science Journal Classification (ASJC) codes
- Aquatic Science
- Animal Science and Zoology
これを引用
Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of “new ulcer disease⇝ in koi carp. / Somamoto, Tomonori; Maruyama, Shinpei; Nagasawa, Takahiro; Nakao, Miki; Sato, Atsushi; Hatta, Hajime; Sato, Mitsuru; Murakami-Yamaguchi, Yukie; Kizu-Mori, Kumiko; Hirakawa, Yuki; Narita, Hiroshi.
：: Fish Pathology, 巻 53, 番号 1, 01.01.2018, p. 36-39.研究成果: ジャーナルへの寄稿 › 記事
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TY - JOUR
T1 - Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of “new ulcer disease⇝ in koi carp
AU - Somamoto, Tomonori
AU - Maruyama, Shinpei
AU - Nagasawa, Takahiro
AU - Nakao, Miki
AU - Sato, Atsushi
AU - Hatta, Hajime
AU - Sato, Mitsuru
AU - Murakami-Yamaguchi, Yukie
AU - Kizu-Mori, Kumiko
AU - Hirakawa, Yuki
AU - Narita, Hiroshi
PY - 2018/1/1
Y1 - 2018/1/1
N2 - To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromo-nads.
AB - To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromo-nads.
UR - http://www.scopus.com/inward/record.url?scp=85045944656&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045944656&partnerID=8YFLogxK
U2 - 10.3147/jsfp.53.36
DO - 10.3147/jsfp.53.36
M3 - Article
AN - SCOPUS:85045944656
VL - 53
SP - 36
EP - 39
JO - Fish Pathology
JF - Fish Pathology
SN - 0388-788X
IS - 1
ER -