Development of fluorogenic probes for quick No-Wash live-cell imaging of intracellular proteins

Yuichiro Hori, Tomoya Norinobu, Motoki Sato, Kyohei Arita, Masahiro Shirakawa, Kazuya Kikuchi

研究成果: ジャーナルへの寄稿学術誌査読

87 被引用数 (Scopus)

抄録

We developed novel fluorogenic probes for no-wash live-cell imaging of proteins fused to PYP-tag, which is a small protein tag recently reported by our group. Through the design of a new PYP-tag ligand, specific intracellular protein labeling with rapid kinetics and fluorogenic response was accomplished. The probes crossed the cell membrane, and cytosolic and nuclear localizations of PYP-tagged proteins without cell washing were visualized within a 6-min reaction time. The fluorogenic response was due to the environmental effect of fluorophore upon binding to PYP-tag. Furthermore, the PYP-tag-based method was applied to the imaging of methyl-CpG-binding domain localization. This rapid protein-labeling system combined with the small protein tag and designed fluorogenic probes offers a powerful method to study the localization, movement, and function of cellular proteins.

本文言語英語
ページ(範囲)12360-12365
ページ数6
ジャーナルJournal of the American Chemical Society
135
33
DOI
出版ステータス出版済み - 8月 21 2013
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 触媒
  • 化学 (全般)
  • 生化学
  • コロイド化学および表面化学

フィンガープリント

「Development of fluorogenic probes for quick No-Wash live-cell imaging of intracellular proteins」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル