Development of high-sensitive cytotoxic detection system using HSP70B promoter

K. I. Wada, L. Xu, T. Okano, A. Taniguchi

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

The development of a high-sensitive cytotoxic detection system using heat-shock proteins (HSP)70B promoter was discussed. Human liver carcinoma cell line was grown in DMEM at 37 °C in a humidified atmosphere with 5% CO 2. Cell viability was evaluated by quantification of DNA performed by using Cyquant Proliferation Assay kit. Subsequent sequencing revealed that transcriptional starting site was mapped at - 110 bp from the translational starting site in HepG2. These results suggests that the effect of low concentration of cadmium chloride on cytotoxicity could be detected by luciferase analysis using a part of HASP70B promoter.

本文言語英語
ホスト出版物のタイトルTransactions - 7th World Biomaterials Congress
ページ1248
ページ数1
出版ステータス出版済み - 2004
外部発表はい
イベントTransactions - 7th World Biomaterials Congress - Sydney, オーストラリア
継続期間: 5 17 20045 21 2004

出版物シリーズ

名前Transactions - 7th World Biomaterials Congress

その他

その他Transactions - 7th World Biomaterials Congress
国/地域オーストラリア
CitySydney
Period5/17/045/21/04

All Science Journal Classification (ASJC) codes

  • 工学(全般)

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