Development of high-speed and highly efficient butanol production systems from butyric acid with high density of living cells of Clostridium saccharoperbutylacetonicum

Shun ichi Baba, Yukihiro Tashiro, Hideaki Shinto, Kenji Sonomoto

研究成果: Contribution to journalArticle査読

33 被引用数 (Scopus)

抄録

Living cells are alive and have the butanol-producing ability but not much proliferation under nitrogen source-limited condition. We investigated various butanol production systems with high density of living cells of Clostridium saccharoperbutylacetonicum N1-4 supplemented with methyl viologen (MV) as an electron carrier and nutrient dosing for activity regeneration. In continuous butanol production with high density of living cells, butanol yield was drastically increased from 0.365C-mol/C-mol with growing cells to 0.528C-mol/C-mol at a dilution rate of 0.85h -1, being increased with the butanol to total solvent ratio. This yield was increased to 0.591C-mol/C-mol by adding 0.01mM MV. MV addition increased not only butanol yield but also butanol concentration and productivity as compared to those without MV addition. However, living cells lost their activity with incubation time, which lowered the operational stability of the system. Therefore, to maintain constant stability, activity regeneration was carried out with high density of living cells and MV. This system produced butanol at high concentration (9.40gl -1) and productivity (7.99gl -1h -1) for approximately 100h with maintenance of considerably high yield of butanol (0.686C-mol/C-mol). Thus, we established a high-speed and highly efficient butanol production system.

本文言語英語
ページ(範囲)605-612
ページ数8
ジャーナルJournal of Biotechnology
157
4
DOI
出版ステータス出版済み - 2 20 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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