Development of highly functional long-term culture method of liver slice embedded in agarose gel for bioartificial liver

Hideki Nonaka, Hirohiko Ise, Nobuhiro Sugihara, Shinichi Hirose, Naoki Negishi, Yoshiyuki Kondo, Toshihiro Akaike

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

It is difficult to a produce highly functional bioartificial liver (BAL) using only hepatocytes, because it is believed that liver-specific three-dimensional structure is necessary to maintain high function for BAL. But it is difficult to construct a culture system with liver-specific three-dimensional structure in vitro. To realize a highly functional culture system with liver-specific three-dimensional structure, we developed a culture system using liver slices that keep liver-specific architecture, such as liver lobule and hepatic microvascular system. Liver slices were embedded in agarose gel to maintain them under a moist and three-dimensional environment. We examined the viability and function of liver slices by using various shapes of agarose gel. Liver slices were cultured 1) under stationary condition (control), 2) directly embedded in gel, and 3) embedded in cylindrical gel for good drainage of medium and ventilation of air. The viability and function of the incubated liver slices were evaluated by LDH leakage, histomorphology, and immunohistochemistry. At 10 days, the morphological condition and function of liver slices embedded in cylindrical gel were maintained better than liver slices directly embedded in gel or in the stationary condition. We suggest that high functionality and morphological condition of liver slices could be maintained by embedding in cylindrical gel. In the future, it is possible that this method could be used to develop a highly functional bioartificial liver.

本文言語英語
ページ(範囲)491-498
ページ数8
ジャーナルCell Transplantation
12
5
DOI
出版ステータス出版済み - 2003
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生体医工学
  • 細胞生物学
  • 移植

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