Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems

Masayasu Taki, Shohei Iyoshi, Akio Ojida, Itaru Hamachi, Yukio Yamamoto

研究成果: ジャーナルへの寄稿記事

147 引用 (Scopus)

抄録

We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of CuI present in living cells.

元の言語英語
ページ(範囲)5938-5939
ページ数2
ジャーナルJournal of the American Chemical Society
132
発行部数17
DOI
出版物ステータス出版済み - 5 5 2010
外部発表Yes

Fingerprint

Fluorescent Dyes
Copper
Fluorescein
Reaction products
Ether
Amines
Glutathione
Ethers
Visualization
Fluorescence
Ligands
Cells
Ions
Membranes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

これを引用

Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems. / Taki, Masayasu; Iyoshi, Shohei; Ojida, Akio; Hamachi, Itaru; Yamamoto, Yukio.

:: Journal of the American Chemical Society, 巻 132, 番号 17, 05.05.2010, p. 5938-5939.

研究成果: ジャーナルへの寄稿記事

Taki, Masayasu ; Iyoshi, Shohei ; Ojida, Akio ; Hamachi, Itaru ; Yamamoto, Yukio. / Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems. :: Journal of the American Chemical Society. 2010 ; 巻 132, 番号 17. pp. 5938-5939.
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