Development of LHRH-a Copolymer Pellet Polymerized by Ultraviolet and Its Application for Maturation in Red Sea Bream Pagrus major during the Non-Spawning Season

Michiya Matsuyama, Minoru Hamada, Taisuke Ashitani, Koichi Okuzawa, Hirohiko Kagawa, Hideki Tanaka, Toshio Iwai, Masaaki Kashiwagi

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

A method is described for preparing a nonbiodegradable copolymer pellet containing luteinizing hormone-releasing hormone analogue (LHRH-a), des Gly10-[D~Ala6] LHRH ethyl-amide, polymerized by ultraviolet rays to produce sustained hormone release. The pellet was applied to induce gonadal development and maturation in red sea bream Pagrus major during the non-spawning season, from November to December. Single implantation of a copolymer pellet containing 100 jug LHRH-a induced gonadal development and spawning in completely immature fish 18 days after implantation, about six months prior to the nutural spawning season. Fish implanted with a 50 fig LHRH-a copolymer pellet or a control pellet were not induced to spawn. The histopathological findings of muscular tissues surrounding the implant showed that no inflammatory response to a foreign body appeared even one month after implantation. Results from the present study indicate that the LHRH-a copolymer pellets prepared by us have several features: (1) the pellets can be prepared easily and mass-produced at one time, (2) the pellets are useful for long-term hormone therapy in inducing sexual maturation in fish requiring a series of hormone treatments, and (3) the copolymer has good biocompatibility. However, the fertility rates of eggs produced were low. Further investigation is necessary to devise an effective method for obtaining more viable eggs by the use of copolymer pellets.

元の言語英語
ページ(範囲)1361-1369
ページ数9
ジャーナルNIPPON SUISAN GAKKAISHI
59
発行部数8
DOI
出版物ステータス出版済み - 1 1 1993
外部発表Yes

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synthetic hormones
Pagrus major
composite polymers
gonadotropin-releasing hormone
hormone
pellets
maturation
hormones
spawning
fish
biocompatibility
sea
foreign bodies
egg
figs
amides
ultraviolet radiation
inflammation
immatures
fertility

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Development of LHRH-a Copolymer Pellet Polymerized by Ultraviolet and Its Application for Maturation in Red Sea Bream Pagrus major during the Non-Spawning Season. / Matsuyama, Michiya; Hamada, Minoru; Ashitani, Taisuke; Okuzawa, Koichi; Kagawa, Hirohiko; Tanaka, Hideki; Iwai, Toshio; Kashiwagi, Masaaki.

:: NIPPON SUISAN GAKKAISHI, 巻 59, 番号 8, 01.01.1993, p. 1361-1369.

研究成果: ジャーナルへの寄稿記事

Matsuyama, Michiya ; Hamada, Minoru ; Ashitani, Taisuke ; Okuzawa, Koichi ; Kagawa, Hirohiko ; Tanaka, Hideki ; Iwai, Toshio ; Kashiwagi, Masaaki. / Development of LHRH-a Copolymer Pellet Polymerized by Ultraviolet and Its Application for Maturation in Red Sea Bream Pagrus major during the Non-Spawning Season. :: NIPPON SUISAN GAKKAISHI. 1993 ; 巻 59, 番号 8. pp. 1361-1369.
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abstract = "A method is described for preparing a nonbiodegradable copolymer pellet containing luteinizing hormone-releasing hormone analogue (LHRH-a), des Gly10-[D~Ala6] LHRH ethyl-amide, polymerized by ultraviolet rays to produce sustained hormone release. The pellet was applied to induce gonadal development and maturation in red sea bream Pagrus major during the non-spawning season, from November to December. Single implantation of a copolymer pellet containing 100 jug LHRH-a induced gonadal development and spawning in completely immature fish 18 days after implantation, about six months prior to the nutural spawning season. Fish implanted with a 50 fig LHRH-a copolymer pellet or a control pellet were not induced to spawn. The histopathological findings of muscular tissues surrounding the implant showed that no inflammatory response to a foreign body appeared even one month after implantation. Results from the present study indicate that the LHRH-a copolymer pellets prepared by us have several features: (1) the pellets can be prepared easily and mass-produced at one time, (2) the pellets are useful for long-term hormone therapy in inducing sexual maturation in fish requiring a series of hormone treatments, and (3) the copolymer has good biocompatibility. However, the fertility rates of eggs produced were low. Further investigation is necessary to devise an effective method for obtaining more viable eggs by the use of copolymer pellets.",
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T1 - Development of LHRH-a Copolymer Pellet Polymerized by Ultraviolet and Its Application for Maturation in Red Sea Bream Pagrus major during the Non-Spawning Season

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AU - Hamada, Minoru

AU - Ashitani, Taisuke

AU - Okuzawa, Koichi

AU - Kagawa, Hirohiko

AU - Tanaka, Hideki

AU - Iwai, Toshio

AU - Kashiwagi, Masaaki

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