The chemiluminescence (CL) assay measures the ability of a phagocyte to produce light. Milk CL assay can detect an early stage of mastitis precisely because phagocytic leukocytes, such as neutrophils, accumulate in milk as a protective initial reaction after bacterial invasion. Based on the emission of CL by phagocytic leukocytes in milk, we developed a method to diagnose mastitis at an early stage.
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