Development of Multiple Layers of PLCL Scaffold for Vascular Tissue Engineering

Azizah Intan Pangesty, Mitsugu Todo

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

抄録

Cardiovascular disease remains to be the major cause of death in Indonesia by 2018. Treatment to replace the ruptured blood vessel by a bypass surgery is often limited by the availability of autologous vascular, while the use of synthetic conduit is challenged by the mechanical mismatch and foreign body reactions. Tissue engineering offers a new approach to create artificial blood vessel with growth potential similar to the native tissue. This study aimed to develop the multiple layers of the cylindrical scaffold made of poly (lactic acid-co-ϵ-caprolactone) (PLCL) by the phase separation and the freeze drying method. The three types of scaffolds were fabricated: single layer, double and triple layer scaffold. The effect to the microstructural behaviour was observed using FESEM. The mechanical properties were characterized using ring tensile test. The biological properties including cell attachment and proliferation using endothelial cells (ECs) were evaluated during one week of culture. SEM observation revealed that the pore size increased as the number of layer increased. Single, double and triple layers of scaffolds had the average pore size of 219 μm 2 , 744 μm 2 and 684 μm 2 , respectively. Meanwhile, the porosity significantly decreased with the increase number of layers. The porosity of single, double and triple layer scaffolds were 92.8%, 68.9 %, and 64.9%, respectively. As the porosity decreased, the mechanical properties including elastic modulus, tensile strength and burst pressure improved in significant amount. The elastic modulus increased 2 folds from the single layer (0.98 MPa), double layers (7.36 MPa) and triple layers (15.45 MPa) scaffolds. The tensile strength of single, double and triple layer scaffold were 216 kPa, 1039 kPa, and 1453 kPa, respectively. The burst pressure of the single layer scaffold was 119 mmHg and increased in double layers and triple layers scaffolds, 305 mmHg and 604 mmHg, respectively. An increased of cell proliferation on the PLCL scaffold during one week of culture indicated that the scaffold is biocompatible for tissue regeneration. This study demonstrated that the mechanical properties can be controlled by creating multiple layers of the cylindrical scaffold. The double and triple layers cylindrical scaffolds are potential candidates for vascular tissue engineering application.

本文言語英語
ホスト出版物のタイトルProceedings of 2018 2nd International Conference on Biomedical Engineering
ホスト出版物のサブタイトルSmart Technology for Better Society, IBIOMED 2018
出版社Institute of Electrical and Electronics Engineers Inc.
ページ138-142
ページ数5
ISBN(電子版)9781538647363
DOI
出版ステータス出版済み - 11月 13 2018
イベント2nd International Conference on Biomedical Engineering, IBIOMED 2018 - Kuta, Bali, インドネシア
継続期間: 7月 24 20187月 26 2018

出版物シリーズ

名前Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018

その他

その他2nd International Conference on Biomedical Engineering, IBIOMED 2018
国/地域インドネシア
CityKuta, Bali
Period7/24/187/26/18

!!!All Science Journal Classification (ASJC) codes

  • コンピュータ サイエンスの応用
  • 生体医工学
  • 健康情報学
  • 器械工学

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