Development of polyethyleneglycol monolayer-based SPR immunosensor for detection of low-molecular-weight fragrants

K. Vengatajalabathy Gobi, Hidekazu Ikezaki, Norio Miura, Kiyoshi Matsumoto, Kiyoshi Toko

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

抄録

We report our investigations on the development of a novel self-assembled monolayer (SAM)-based flow-through surface-plasmon-resonance (SPR) immunosensor for highly specific and rapid detection of benzaldehyde (BZ). The functional sensing surface was immobilized with a BZ derivative on the SAM of polyethyleneglycol-alkanethiols. Both the selfassembling of alkanethiols and the immobilization of BZ were controlled by the analysis of SPR resonance angle measurements. The BZ-bound SAM surface shows high specificity for immunoaffinity reaction with anti-BZ antibody (BZ-Ab). The quantification of benzaldehyde is based on the principle of indirect competitive immunoassay, in which the immunoreaction between the BZ derivative on sensor surface and anti-BZ antibody was inhibited in the presence of free BZ in solution. The decrease in SPR angle is proportional to the increase in the concentration of BZ in test samples. The SPR immunosensor exhibited excellent sensitivity for the detection of BZ in the concentration range from 0.1-80 ppb (ng ml-1). Detection limit of the present sensor is superior compared to various high-performance SPR and QCM sensors. Similarly, HPLC and GC techniques reported a low-detection-limit of only 10 ppb BZ, despite a set of sample pretreatments. In addition, enhancement of the SPR sensor signal and the sensitivity were investigated by introducing a single-step add-on strategy involving either Au nanoparticles or a simple biotin-avidin coupling reaction, with which the detection limit has been improved dramatically.

本文言語英語
ホスト出版物のタイトルThe 6th IEEE Conference on SENSORS, IEEE SENSORS 2007
ページ686-689
ページ数4
DOI
出版ステータス出版済み - 2007
イベント6th IEEE Conference on SENSORS, IEEE SENSORS 2007 - Atlanta, GA, 米国
継続期間: 10月 28 200710月 31 2007

出版物シリーズ

名前Proceedings of IEEE Sensors

その他

その他6th IEEE Conference on SENSORS, IEEE SENSORS 2007
国/地域米国
CityAtlanta, GA
Period10/28/0710/31/07

!!!All Science Journal Classification (ASJC) codes

  • 電子工学および電気工学

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