Development of surface plasmon resonance immunosensor for the determination of methyl parathion

Mayumi Tanaka, Kazuhira Sakamoto, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Duck Hwa Chung, Toshihiko Imato

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

A surface plasmon resonance (SPR) sensor for the determination of methyl parathion (MP) was developed using an indirect competitive immunoassay. A sensor chip was prepared by immobilizing a MP-BSA conjugate on a gold film on the sensor chip, followed by blocking with BSA to prevent the non-specific adsorption of MP and an anti-MP antibody onto the unmodified surface of the sensor chip. An evaluation of the adsorption equilibrium of MP-BSA onto the sensor chip by measuring the SPR angle shift revealed that the adsorption equilibrium was expressed by a Langmuir-type adsorption isotherm equation. The adsorption constant and the adsorbed amount of MP-BSA onto the sensor chip were found to be 5.0 × 10 5 M -1 and 0.95 ng/mm 2, respectively. An MP solution (1∼5000 ppb) containing 60 ppm anti-MP antibody was introduced into the SPR sensor chip, and then the SPR angle shift was measured. The detection limit, defined as the angle shift of 85% for the blank, was found to be 10 ppb. The anti-MP antibody bound to the MP-BSA conjugate on the sensor chip was able to be dissociated by introducing an HCl-glycine solution (pH 2) into the sensor chip. Duplicate measurements with a single sensor chip were possible for at least 20 times.

本文言語英語
ページ(範囲)705-712
ページ数8
ジャーナルbunseki kagaku
56
9
DOI
出版ステータス出版済み - 11 21 2007

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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