TY - CHAP
T1 - Development of triplex forming oligonucleotide including artificial nucleoside analogues for the antigene strategy
AU - Taniguchi, Yosuke
AU - Sasaki, Shigeki
PY - 2018/12/8
Y1 - 2018/12/8
N2 - The sequence-specific triplex formation against duplex DNA offers a potential basis for genome targeting technology, such as diagnostics, regulation of gene expression and sequencing technologies. In an antiparallel triplex DNA, a purine-rich triplex forming oligonucleotide (TFO) consisting of a dG, dA or T forms two reverse Hoogsteen hydrogen bonds with a GC, AT or AT base pair of the duplex DNA, respectively, with a high selectivity in a sequence specific manner. However, there is no natural nucleoside which can recognize the inverted CG and TA base pair of the duplex DNA. Therefore, the development of recognition molecules for the CG and TA inversion sites with a high stability and selectivity has been demanded for the triplex forming technology. In this chapter, we describe the design and synthesis of W-shaped nucleoside analogues (WNA-βT) and pseudo-dC derivatives (MeAP-ΨdC) for selective recognition of the TA and CG base pair, respectively, to expand the triplex-forming sequence.
AB - The sequence-specific triplex formation against duplex DNA offers a potential basis for genome targeting technology, such as diagnostics, regulation of gene expression and sequencing technologies. In an antiparallel triplex DNA, a purine-rich triplex forming oligonucleotide (TFO) consisting of a dG, dA or T forms two reverse Hoogsteen hydrogen bonds with a GC, AT or AT base pair of the duplex DNA, respectively, with a high selectivity in a sequence specific manner. However, there is no natural nucleoside which can recognize the inverted CG and TA base pair of the duplex DNA. Therefore, the development of recognition molecules for the CG and TA inversion sites with a high stability and selectivity has been demanded for the triplex forming technology. In this chapter, we describe the design and synthesis of W-shaped nucleoside analogues (WNA-βT) and pseudo-dC derivatives (MeAP-ΨdC) for selective recognition of the TA and CG base pair, respectively, to expand the triplex-forming sequence.
UR - http://www.scopus.com/inward/record.url?scp=85079566194&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079566194&partnerID=8YFLogxK
U2 - 10.1007/978-981-13-1912-9_15
DO - 10.1007/978-981-13-1912-9_15
M3 - Chapter
SN - 9789811319112
SP - 253
EP - 269
BT - Synthesis of Therapeutic Oligonucleotides
PB - Springer Singapore
ER -