Developmental changes in myoendothelial gap junction mediated vasodilator activity in the rat saphenous artery

Shaun L. Sandow, Kenichi Goto, Nicole M. Rummery, Caryl E. Hill

研究成果: ジャーナルへの寄稿記事

54 引用 (Scopus)

抄録

A role for myoendothelial gap junctions (MEGJs) has been proposed in the action of the vasodilator endothelium-derived hyperpolarizing factor (EDHF). EDHF activity varies in disease and during ageing, but little is known of the role of EDHF during development when, in many organ systems, gap junctions are up-regulated. The aims of the present study were therefore to determine whether an up-regulation of heterocellular gap junctional coupling occurs during arterial development and whether this change is reflected functionally through an increased action of EDHF. Results demonstrated that in the saphenous artery of juvenile WKY rats, MEGJs were abundant and application of acetylcholine (ACh) evoked EDHF-mediated hyperpolarization and relaxation in the presence of Nω-nitro-L-arginine methyl ester (L-NAME) and indomethacin to inhibit nitric oxide and prostaglandins, respectively. Responses were blocked by a combination of charybdotoxin plus apamin, or 1-[(2-chlorophenyl)diphenylmethyl]-1H-pyrazole (TRAM-34) plus apamin, or by blockade of gap junctions with the connexin (Cx-mimetic peptides, 43Gap26, 40Gap27 and 37,43Gap27. On the other hand, we found no evidence for the involvement of the putative chemical mediators of EDHF, eicosanoids, L-NAME-insensitive nitric oxide, hydrogen peroxide or potassium ions, since 14,15-epoxyeicosa-5(Z)-enoic acid (14,15-EEZE), hydroxocobalamin, catalase or barium and ouabain were without effect. In contrast, in the adult saphenous artery, MEGJs were rare, EDHF-mediated relaxation was absent and hyperpolarizations were small and unstable. The present study demonstrates that MEGJs and EDHF are up-regulated during arterial development. Furthermore, the data show for the first time that this developmentally regulated EDHF is dependent on direct electrotonic coupling via MEGJs.

元の言語英語
ページ(範囲)875-886
ページ数12
ジャーナルJournal of Physiology
556
発行部数3
DOI
出版物ステータス出版済み - 5 1 2004
外部発表Yes

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Gap Junctions
Vasodilator Agents
Endothelium
Arteries
Apamin
NG-Nitroarginine Methyl Ester
Nitric Oxide
Hydroxocobalamin
Charybdotoxin
Connexins
Inbred WKY Rats
Eicosanoids
Ouabain
Barium
Indomethacin
Catalase
Hydrogen Peroxide
Acetylcholine
Prostaglandins
Potassium

All Science Journal Classification (ASJC) codes

  • Physiology

これを引用

Developmental changes in myoendothelial gap junction mediated vasodilator activity in the rat saphenous artery. / Sandow, Shaun L.; Goto, Kenichi; Rummery, Nicole M.; Hill, Caryl E.

:: Journal of Physiology, 巻 556, 番号 3, 01.05.2004, p. 875-886.

研究成果: ジャーナルへの寄稿記事

Sandow, Shaun L. ; Goto, Kenichi ; Rummery, Nicole M. ; Hill, Caryl E. / Developmental changes in myoendothelial gap junction mediated vasodilator activity in the rat saphenous artery. :: Journal of Physiology. 2004 ; 巻 556, 番号 3. pp. 875-886.
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