Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths

Zhenhao Ding, Hiromichi Kasahara, Michihiko Nakano, Junya Suehiro

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

Polymerase chain reaction (PCR) is one of the most sensitive and specific detection methods of bacterial and viral infections. The authors proposed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labelling. DNA labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. DEP-trapped microbeads can be measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. In this study, it was aimed to reveal how DNA length affects DEP characteristic of DNA-labeled microbeads. Dielectrophoretic crossover from the negative to the positive was measured for microbeads labeled with DNA length in 204 bp, 391 bp and 796 bp. After theoretical fitting of DEP crossover data, it was revealed that the surface conductance increased when the length of labeled DNA increased.

元の言語英語
ホスト出版物のタイトルBIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015
編集者Alberto Cliquet, Ana Fred, Hugo Gamboa, Dirk Elias, Alberto Cliquet
出版者SciTePress
ページ185-189
ページ数5
ISBN(電子版)9789897580710
出版物ステータス出版済み - 1 1 2015
イベント8th International Conference on Biomedical Electronics and Devices, BIODEVICES 2015 - Lisbon, ポルトガル
継続期間: 1 12 20151 15 2015

出版物シリーズ

名前BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015

その他

その他8th International Conference on Biomedical Electronics and Devices, BIODEVICES 2015
ポルトガル
Lisbon
期間1/12/151/15/15

Fingerprint

DNA
Electrophoresis
Polymerase chain reaction
Microelectrodes
Labeling

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Electrical and Electronic Engineering

これを引用

Ding, Z., Kasahara, H., Nakano, M., & Suehiro, J. (2015). Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. : A. Cliquet, A. Fred, H. Gamboa, D. Elias, & A. Cliquet (版), BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015 (pp. 185-189). (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015). SciTePress.

Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. / Ding, Zhenhao; Kasahara, Hiromichi; Nakano, Michihiko; Suehiro, Junya.

BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. 版 / Alberto Cliquet; Ana Fred; Hugo Gamboa; Dirk Elias; Alberto Cliquet. SciTePress, 2015. p. 185-189 (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015).

研究成果: 著書/レポートタイプへの貢献会議での発言

Ding, Z, Kasahara, H, Nakano, M & Suehiro, J 2015, Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. : A Cliquet, A Fred, H Gamboa, D Elias & A Cliquet (版), BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015, SciTePress, pp. 185-189, 8th International Conference on Biomedical Electronics and Devices, BIODEVICES 2015, Lisbon, ポルトガル, 1/12/15.
Ding Z, Kasahara H, Nakano M, Suehiro J. Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. : Cliquet A, Fred A, Gamboa H, Elias D, Cliquet A, 編集者, BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. SciTePress. 2015. p. 185-189. (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015).
Ding, Zhenhao ; Kasahara, Hiromichi ; Nakano, Michihiko ; Suehiro, Junya. / Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths. BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015. 編集者 / Alberto Cliquet ; Ana Fred ; Hugo Gamboa ; Dirk Elias ; Alberto Cliquet. SciTePress, 2015. pp. 185-189 (BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015).
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