TY - JOUR
T1 - Dietary Intake of Curcumin Improves eIF2 Signaling and Reduces Lipid Levels in the White Adipose Tissue of Obese Mice
AU - Kobori, Masuko
AU - Takahashi, Yumiko
AU - Takeda, Hiroaki
AU - Takahashi, Masatomo
AU - Izumi, Yoshihiro
AU - Akimoto, Yukari
AU - Sakurai, Mutsumi
AU - Oike, Hideaki
AU - Nakagawa, Toshiyuki
AU - Itoh, Masanori
AU - Bamba, Takeshi
AU - Kimura, Toshiyuki
N1 - Funding Information:
This work was financially supported in part by a grant from a Ministry of Agriculture, Forestry and Fisheries (MAFF) research project for the special scheme to deploy highly advanced technology for agriculture, forestry, and fisheries (to M.K. and T.B.), a grant from the Research Project on Development of Agricultural Products and Foods with Health-Promoting Benefits, National Agriculture and Food Research Organization (NARO) (to M.K.), Ministry of Education, Culture, Sports, Science and Technology, Japan (15K00867 to M.K.), the Uragami Foundation (to M.K.), a grant from the Advanced low carbon technology research and development program (ALCA) of the Japan Science and Technolocy Agency (JST) (to Y.I. and T.B.), a grant from the Studies on Specific Activities and Functions of Lipid Molecules to Develop Innovative Medical Technologies Project of the Japan Agency for Medical Research and Development (AMED) (to Y.I. and T.B.), and a grant from the Creation of Innovative Technology for Medical Applications Based on the Global Analyses and Regulation of Disease-Related Metabolites Project (JPMJCR1395) of the AMED (to T.B.). We thank Dr. Hiroshi Ono and Ms. Tomoko Sato for performing the UHPLC-ESI-Q-Orbitrap-MS analysis at Advanced Analysis Center, NARO. We thank Dr. Ohnishi-Kameyama (Food Research Institute, NARO), for valuable advice. We thank Enago (Crimson Interactive Japan, Tokyo, Japan) for the English language review.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - White adipose tissue (eWAT) plays a crucial role in preventing metabolic syndrome. We aimed to investigate WAT distribution and gene expression and lipidomic profiles in epididymal WAT (eWAT) in diet-induced obese mice, reflecting a Western-style diet of humans to elucidate the bioactive properties of the dietary antioxidant curcumin in preventing lifestyle-related diseases. For 16 weeks, we fed C57BL/6J mice with a control diet, a high-fat, high-sucrose and high-cholesterol Western diet or Western diet supplemented with 0.1% (w/w) curcumin. Although the dietary intake of curcumin did not affect eWAT weight or plasma lipid levels, it reduced lipid peroxidation markers' levels in eWAT. Curcumin accumulated in eWAT and changed gene expressions related to eukaryotic translation initiation factor 2 (eIF2) signalling. Curcumin suppressed eIF2α phosphorylation, which is induced by endoplasmic reticulum (ER) stress, macrophage accumulation and nuclear factor-κB (NF-κB) p65 and leptin expression, whereas it's anti-inflammatory effect was inadequate to decrease TNF-α and IFN-γ levels. Lipidomic and gene expression analysis revealed that curcumin decreased some diacylglycerols (DAGs) and DAG-derived glycerophospholipids levels by suppressing the glycerol-3-phosphate acyltransferase 1 and adipose triglyceride lipase expression, which are associated with lipogenesis and lipolysis, respectively. Presumably, these intertwined effects contribute to metabolic syndrome prevention by dietary modification.
AB - White adipose tissue (eWAT) plays a crucial role in preventing metabolic syndrome. We aimed to investigate WAT distribution and gene expression and lipidomic profiles in epididymal WAT (eWAT) in diet-induced obese mice, reflecting a Western-style diet of humans to elucidate the bioactive properties of the dietary antioxidant curcumin in preventing lifestyle-related diseases. For 16 weeks, we fed C57BL/6J mice with a control diet, a high-fat, high-sucrose and high-cholesterol Western diet or Western diet supplemented with 0.1% (w/w) curcumin. Although the dietary intake of curcumin did not affect eWAT weight or plasma lipid levels, it reduced lipid peroxidation markers' levels in eWAT. Curcumin accumulated in eWAT and changed gene expressions related to eukaryotic translation initiation factor 2 (eIF2) signalling. Curcumin suppressed eIF2α phosphorylation, which is induced by endoplasmic reticulum (ER) stress, macrophage accumulation and nuclear factor-κB (NF-κB) p65 and leptin expression, whereas it's anti-inflammatory effect was inadequate to decrease TNF-α and IFN-γ levels. Lipidomic and gene expression analysis revealed that curcumin decreased some diacylglycerols (DAGs) and DAG-derived glycerophospholipids levels by suppressing the glycerol-3-phosphate acyltransferase 1 and adipose triglyceride lipase expression, which are associated with lipogenesis and lipolysis, respectively. Presumably, these intertwined effects contribute to metabolic syndrome prevention by dietary modification.
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U2 - 10.1038/s41598-018-27105-w
DO - 10.1038/s41598-018-27105-w
M3 - Article
C2 - 29899429
AN - SCOPUS:85048430250
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 9081
ER -