TY - JOUR
T1 - Difference in the antibacterial action of epigallocatechin gallate and theaflavin 3,3'-di-O-gallate on Bacillus coagulans
AU - Sato, J.
AU - Nakayama, M.
AU - Tomita, A.
AU - Sonoda, T.
AU - Miyamoto, T.
N1 - Funding Information:
This work was supported by Kao Corporation in the form of salaries to the authors, J.S., M.N. A.T. and T.S., in accordance to a contract of collaborative study with T.M., Kyushu University. T.M. was involved in reviewing and editing of the manuscript. However, the funder had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
Publisher Copyright:
© 2020 The Society for Applied Microbiology
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Aims: To study the mechanism of the antibacterial action of tea polyphenols such as catechins and theaflavins against Bacillus coagulans, and the interaction of epigallocatechin gallate (EGCg) or theaflavin 3,3'-di-O-gallate (TFDG) with the surface of B. coagulans cells was investigated. Methods and Results: The antibacterial activities of EGCg and TFDG against B. coagulans cells were measured by counting of the viable cells after the mixing with each polyphenol. Bactericidal effect of TFDG was shown at the concentration of greater than or equal to 62·5 mg l−1; however, at the same concentration, EGCg did not. According to the results of two dimensional (2D)-electrophoresis analysis, TFDG seemed to interact with cytoplasmic membrane proteins. The activity of the glucose transporters of the cells decreased 40% following the treatment with TFDG of 62·5 mg l−1; however, this decrease was only slight in case of EGCg. This result was in accordance with the strength of their bactericidal activities. Conclusion: Our results suggest that the direct interaction between membrane proteins and TFDG is an important factor in the antibacterial activity of polymerized catechins, affecting their functions and leading to cell death. Significance and Impact of the Study: Tea polyphenols can effectively use the prevention of product spoilage in the food and beverage industry.
AB - Aims: To study the mechanism of the antibacterial action of tea polyphenols such as catechins and theaflavins against Bacillus coagulans, and the interaction of epigallocatechin gallate (EGCg) or theaflavin 3,3'-di-O-gallate (TFDG) with the surface of B. coagulans cells was investigated. Methods and Results: The antibacterial activities of EGCg and TFDG against B. coagulans cells were measured by counting of the viable cells after the mixing with each polyphenol. Bactericidal effect of TFDG was shown at the concentration of greater than or equal to 62·5 mg l−1; however, at the same concentration, EGCg did not. According to the results of two dimensional (2D)-electrophoresis analysis, TFDG seemed to interact with cytoplasmic membrane proteins. The activity of the glucose transporters of the cells decreased 40% following the treatment with TFDG of 62·5 mg l−1; however, this decrease was only slight in case of EGCg. This result was in accordance with the strength of their bactericidal activities. Conclusion: Our results suggest that the direct interaction between membrane proteins and TFDG is an important factor in the antibacterial activity of polymerized catechins, affecting their functions and leading to cell death. Significance and Impact of the Study: Tea polyphenols can effectively use the prevention of product spoilage in the food and beverage industry.
UR - http://www.scopus.com/inward/record.url?scp=85084137512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084137512&partnerID=8YFLogxK
U2 - 10.1111/jam.14662
DO - 10.1111/jam.14662
M3 - Article
C2 - 32281733
AN - SCOPUS:85084137512
SN - 1364-5072
VL - 129
SP - 601
EP - 611
JO - Proceedings of the Society for Applied Bacteriology
JF - Proceedings of the Society for Applied Bacteriology
IS - 3
ER -