Differential activation of proapoptotic molecules between mouse and rat models of distal motor trigeminal denervation

Shiori Harada, Satoshi O. Suzuki, Yoshihiro Seki, Seiji Nakamura, Toru Iwaki

研究成果: Contribution to journalArticle査読


Background: We previously developed a rat trigeminal motor neuron axotomy model involving masseter and temporal muscle resection to study pathological changes of the central nucleus after peripheral nerve injury caused by oral surgery. Because motor neurons are reported to be more vulnerable to axotomy in mice than rats, we compared the degeneration process of the trigeminal motor nucleus in the rat model with a similar mouse model. Methods: We removed masseter and temporal muscles of adult mice or rats. Animals were sacrificed at 3, 7, 14, 28, 42, and 56days post-operation, and the trigeminal motor nuclei were histologically analyzed. Results: Size reduction, but no neuronal loss, was seen in the trigeminal motor nuclei in both mice and rats. Time-dependent Noxa expression, starting at 1week post-operation (wpo), was seen in the mouse model. By 8wpo, mice expressed a higher level of Noxa than rats. Additionally, we noted persistent expression of cleaved caspase-3 in mice but not in rats. Conversely, apoptosis-inducing factor (AIF), which executes DNA fragmentation in the nucleus, was not translocated to the nucleus in either model. Conclusions: Our findings indicate differential activation of motor neuron apoptosis pathways after axotomy in mice and rats. Lack of activation of caspase-independent pathways and distal end denervation in our model might be related to the survival of motor neurons after axonal injury. These findings could be relevant to future neuroprotective strategies for peripheral nerve injury caused by oral surgeries.

ジャーナルJournal of Oral Pathology and Medicine
出版ステータス出版済み - 4 2012

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 口腔外科
  • 耳鼻咽喉科学
  • 癌研究
  • 歯周病学


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