Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells

Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Takahiro Shiotsuki, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

The silkworm Fanconi anemia (FA) pathway is required for normal cellular resistance to mitomycin C (MMC) in silkworms, but little is known about the requirement for repair of other types of DNA damage. Here we report that silkworm cells deficient for FA proteins FancD2 and FancM exhibit normal sensitivities to hydroxyurea (HU) and camptothecin (CPT), although FancM-dependent FancD2 monoubiquitination is induced upon these treatments. Similar results were observed in cells depleted for Rmi1 and Mhf1, which interact with the FancM protein. We also found that Rad51-knockdown cells exhibited normal sensitivity to HU despite induction of double-strand breaks by HU treatment.

本文言語英語
ページ(範囲)3959-3963
ページ数5
ジャーナルFEBS Letters
588
21
DOI
出版ステータス出版済み - 11 3 2014

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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