Differential cytokine contributions of perivascular haematopoietic stem cell niches

Noboru Asada, Yuya Kunisaki, Halley Pierce, Zichen Wang, Nicolas F. Fernandez, Alexander Birbrair, Avi Ma'ayan, Paul S. Frenette

研究成果: ジャーナルへの寄稿記事

123 引用 (Scopus)


Arterioles and sinusoids of the bone marrow (BM) are accompanied by stromal cells that express nerve/glial antigen 2 (NG2) and leptin receptor (LepR), and constitute specialized niches that regulate quiescence and proliferation of haematopoietic stem cells (HSCs). However, how niche cells differentially regulate HSC functions remains unknown. Here, we show that the effects of cytokines regulating HSC functions are dependent on the producing cell sources. Deletion of chemokine C-X-C motif ligand 12 (Cxcl12) or stem cell factor (Scf) from all perivascular cells marked by nestin-GFP dramatically depleted BM HSCs. Selective Cxcl12 deletion from arteriolar NG2+ cells, but not from sinusoidal LepR+ cells, caused HSC reductions and altered HSC localization in BM. By contrast, deletion of Scf in LepR+ cells, but not NG2+ cells, led to reductions in BM HSC numbers. These results uncover distinct contributions of cytokines derived from perivascular cells in separate vascular niches to HSC maintenance.

ジャーナルNature Cell Biology
出版物ステータス出版済み - 3 1 2017


All Science Journal Classification (ASJC) codes

  • Cell Biology


Asada, N., Kunisaki, Y., Pierce, H., Wang, Z., Fernandez, N. F., Birbrair, A., ... Frenette, P. S. (2017). Differential cytokine contributions of perivascular haematopoietic stem cell niches. Nature Cell Biology, 19(3), 214-223. https://doi.org/10.1038/ncb3475