Differential display analysis of gene expression in developing embryos of Xenopus laevis

Naoki Adati, Takashi Ito, Chie Koga, Keiji Kito, Yoshiyuki Sakaki, Koichiro Shiokawa

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

Differential display (DD), an arbitrarily primed RT-PCR fingerprinting technique, is a novel approach for the search of differentially expressed transcripts. Using our improved DD protocol, reproducible cDNA fingerprints were successfully obtained from RNAs of Xenopus laevis embryos at six representative stages. Parallel comparison among the fingerprints revealed a number of bands with differential expression patterns. Analysis with clones of three randomly chosen bands confirmed that their expression patterns were faithfully reflected on fingerprints, thereby proving the reliability and validity of the approach. Nucleotide sequencing of these clones revealed that one is identical with a known transcript (cardiac actin), the second is a novel developmentally regulated gene showing no significant homology with those reported previously, and the last is a close but unique relative of XK endo B gene showing somewhat different spatial expression pattern. These results indicated that the DD analysis provides a rapid and reliable way for the identification of novel differentially expressed genes as well as a unique 'scope' for the survey of the changes in overall gene expression profiles occurring in the early embryonic development of Xenopus as well as of other organisms.

元の言語英語
ページ(範囲)43-51
ページ数9
ジャーナルBBA - Gene Structure and Expression
1262
発行部数1
DOI
出版物ステータス出版済み - 5 17 1995
外部発表Yes

Fingerprint

Xenopus laevis
Dermatoglyphics
Gene expression
Embryonic Structures
Genes
Display devices
Gene Expression
Clone Cells
Xenopus
Transcriptome
Reproducibility of Results
Embryonic Development
Actins
Nucleotides
Complementary DNA
RNA
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

これを引用

Differential display analysis of gene expression in developing embryos of Xenopus laevis. / Adati, Naoki; Ito, Takashi; Koga, Chie; Kito, Keiji; Sakaki, Yoshiyuki; Shiokawa, Koichiro.

:: BBA - Gene Structure and Expression, 巻 1262, 番号 1, 17.05.1995, p. 43-51.

研究成果: ジャーナルへの寄稿記事

Adati, Naoki ; Ito, Takashi ; Koga, Chie ; Kito, Keiji ; Sakaki, Yoshiyuki ; Shiokawa, Koichiro. / Differential display analysis of gene expression in developing embryos of Xenopus laevis. :: BBA - Gene Structure and Expression. 1995 ; 巻 1262, 番号 1. pp. 43-51.
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AU - Shiokawa, Koichiro

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