Differential display analysis of mutants for the transcription factor Pdr1p regulating multidrug resistance in the budding yeast

Fumihito Miura, Tetsushi Yada, Kenta Nakai, Yoshiyuki Sakaki, Takashi Ito

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

The transcription factor Pdr1p recognizes Pdr1p/Pdr3p-response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain-of-function alleles of PDR1 using a high-throughput fluorescent differential display PCR. Consequently, we identified 20 transcripts apparently regulated by Pdr1p, which are derived from well-known target genes as well as those that have never been described in the context of drug resistance. Intriguingly, among the latter, a previously unrecognized gene bearing a small putative open reading frame preceded by a functional PDRE was found.

本文言語英語
ページ(範囲)103-108
ページ数6
ジャーナルFEBS Letters
505
1
DOI
出版ステータス出版済み - 9 7 2001
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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