Differential display analysis of mutants for the transcription factor Pdr1p regulating multidrug resistance in the budding yeast

Fumihito Miura, Tetsushi Yada, Kenta Nakai, Yoshiyuki Sakaki, Takashi Ito

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

The transcription factor Pdr1p recognizes Pdr1p/Pdr3p-response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain-of-function alleles of PDR1 using a high-throughput fluorescent differential display PCR. Consequently, we identified 20 transcripts apparently regulated by Pdr1p, which are derived from well-known target genes as well as those that have never been described in the context of drug resistance. Intriguingly, among the latter, a previously unrecognized gene bearing a small putative open reading frame preceded by a functional PDRE was found.

元の言語英語
ページ(範囲)103-108
ページ数6
ジャーナルFEBS Letters
505
発行部数1
DOI
出版物ステータス出版済み - 9 7 2001
外部発表Yes

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Saccharomycetales
Multiple Drug Resistance
Bearings (structural)
Yeast
Transcription Factors
Genes
Display devices
Response Elements
Transcriptome
Drug Resistance
Open Reading Frames
Yeasts
Alleles
Cells
Throughput
Polymerase Chain Reaction
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Differential display analysis of mutants for the transcription factor Pdr1p regulating multidrug resistance in the budding yeast. / Miura, Fumihito; Yada, Tetsushi; Nakai, Kenta; Sakaki, Yoshiyuki; Ito, Takashi.

:: FEBS Letters, 巻 505, 番号 1, 07.09.2001, p. 103-108.

研究成果: ジャーナルへの寄稿記事

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AU - Ito, Takashi

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