Differential expression of DNA topoisomerase I gene between CPT-11 acquired-and native-resistant human pancreatic tumor cell lines: Detected by RNA PCR-based quantitation assay

Sachi Takeda, Takao Shimazoe, Keiki Sato, Yoshikazu Sugimoto, Takashi Tsuruo, Akira Kono

研究成果: Contribution to journalArticle査読

33 被引用数 (Scopus)

抄録

RNA PCR quantitation method was developed to determine DNA Topoisomerase I(Topo I)-specific mRNA in order to study its gene expression in CPT-11 sensitive, acquired- or native-resistant human pancreatic tumor cell lines. The results were supported by Northern blotting and Western blotting analyses. Acquired-resistant cells have shown decreased levels of Topo I mRNA, compared with their parental cells. On the contrary, in the wild type cells no correlation was shown between sensitivity and gene expression. On the other specific Topo I activity of the native resistant cell lines was fairly lower than that of sensitive cell lines, suggesting that immunoreactive Tope I protein contains low levels of active form enzyme which could be targets of CPT-11 in these native-resistant ones. Finally, the different mechanisms might be operative between acquired- and native-resistant tumor cells.

本文言語英語
ページ(範囲)618-625
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
184
2
DOI
出版ステータス出版済み - 4 30 1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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