Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy

Yusuke Sato, Motoyuki Shimizu, Wataru Mizunoya, Hiroyuki Wariishi, Ryuichi Tatsumi, Vladimir L. Buchman, Yoshihide Ikeuchi

研究成果: Contribution to journalArticle査読

26 被引用数 (Scopus)

抄録

Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electro-phoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.

本文言語英語
ページ(範囲)1748-1756
ページ数9
ジャーナルBioscience, Biotechnology and Biochemistry
73
8
DOI
出版ステータス出版済み - 2009

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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